Eriocheir sinensiswere analyzed using 10 pairs of microsatellite primers. Ninety-five samples were collected from wild stock in the Yangtze River4A and Sheyang cultured stock in Jiangsu Province. F₄A and F₄B stocks were originated from the animals of national original stock farm ofin Gucheng LakeJiangsu. Sheyang cultured stock were offspring of local cultured crabs. The results showed that the average observed heterozygosities and the average expected heterozygosities 0.726 2 and 0.728 6 respectively. The genetic diversities of selective breeding stocks were higher than that of the wild stock（）the four stocks in the present study had relatively higher genetic diversities）4A and F₄B stocks were allocated in the same clusterand Sheyang cultured stock was allocated in another cluster. It revealed that there was no significant difference in genetic differentiation among selective breeding stocks and wild stock.