吴洋磊(1995–),男,硕士研究生,研究方向为水产养殖.E-mail:2533657619@qq.com
S968
国家重点研发计划项目(2018YFD0901604)； 国家自然科学基金项目(31902395)； 辽宁省高等学校创新团队及创新人才支持计划项目(LT2019003)； 南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402).
为探讨高温和低盐对中间球海胆(Strongylocentrotus intermedius)早期胚胎发育进程的影响, 本研究利用中心复合设计(CCD)和响应曲面分析法(RSM), 开展不同温度(12~26 ℃)和盐度(22~34)对中间球海胆胚胎发育早期进程的联合效应研究, 旨在建立温度和盐度对中间球海胆胚胎发育进程的定量关系模型, 并通过统计优化方法得出温度和盐度的最佳组合。结果显示, 在实验设定的温度和盐度范围内, 随着温度的升高, 中间球海胆早期胚胎发育时间呈现出先缩短后延长的趋势; 随着盐度的降低, 中间球海胆胚胎发育早期时间延长。温度的一次效应、二次效应和盐度的一次效应均显著影响(P<0.05)中间球海胆胚胎发育早期进程; 温度一次项系数的绝对值均大于盐度的一次项系数; 温盐的联合效应对中间球海胆胚胎发育早期进程的影响不显著(P>0.05)。实验建立的 2 细胞期、8 细胞期、16 细胞期、囊胚期、上浮期和四腕幼虫期发育进程模型方程决定系数分别为 0.9576、0.9508、0.9689、0.9932、 0.9681 和 0.9763。模型优化和验证试验得出, 温度 20.47 ℃和盐度 31.46 时, 中间球海胆 2 细胞期、8 细胞期、16 细胞期、囊胚期、上浮期、四腕幼虫期的发育时间最短, 分别为 1.28 h、2.07 h、3.31 h、4.14 h、11.28 h 和 47.31 h。 研究结果表明高温和低盐会延长中间球海胆早期胚胎发育时间。
Using the fertilized eggs and larva of Strongylocentrotus intermedius, the effects of high temperatures and low salinity on the early embryonic development of the S. intermedius were investigated, and the tolerance of larvae at various stages to temperature and salinity were studied. Central Composite Design and Response Surface Methodology were used to study the interactive effects of temperatures (12–26 ℃) and salinity (22–34) on the early embryonic development processes of S. intermedius, with the aim of establishing a quantitative relationship model of temperature and salinity on the development processes of embryos, and then through statistical optimization methods obtain the best combination of temperature and salinity. The results showed that the early embryonic development time of S. intermedius first shortened and then lengthened with temperature increase. With salinity decreasing, the early embryo development of S. intermedius increased. The linear effect and the quadratic effect of temperature, and the linear effect of salinity on the early embryonic development process of S. intermedius were significant (P<0.05). The absolute value of the linear coefficient of temperature was greater than the linear coefficient of salinity. The combined effect of temperature and salinity on the early embryonic development of S. intermedius was not significant (P>0.05). The model equation of the 2-cell, 8-cell, 16-cell, blastula, float, and four-arm pluteus larvae toward temperature and salinity were established, with the coefficients of determination (R2) being 0.9576, 0.9508, 0.9689, 0.9932, 0.9681, and 0.9763, respectively. At a temperature of 20.47 ℃ and a salinity of 31.46, the development time of the 2-cell, 8-cell, 16-cell, blastula, float, and four-arm pluteus larvae of S. intermedius were the shortest, which were 1.28 h, 2.07 h, 3.31 h, 4.14 h, 11.28 h and 47.31 h, respectively. The results indicated that a high temperature and low salinity will prolong the early embryonic development processes of S. intermedius. The findings provide a theoretical basis for the artificial breeding of S. intermedius.
吴洋磊,甄昊,王荦,谭八梅,裴泓霖,丁君,常亚青.温度和盐度对中间球海胆胚胎发育早期进程联合效应[J].中国水产科学,2021,28(2):144-156
WU Yanglei, ZHEN Hao, WANG Luo, TAN Bamei, PEI Honglin, DING Jun, CHANG Yaqing. Interactive effect of temperature and salinity on early embryo development processes of the sea urchin (Strongylocentrotus intermedius)[J]. Journal of Fishery Sciences of China,2021,28(2):144-156
