Purification of discharged aquaculture waste water and nitrogen removal have become essential practices as environment protection policies impose increasingly strict waste water discharge restrictions. To improve the purification efficiency of maricultural waste water, we studied the purification effect of biofilters augmented by different compositions of 3 nitrogen-removing bacteria strains on static and flowing maricultural waste water. Firstly, different compositions of 3 nitrogen-removing bacteria strains, Bacillus hwajinpoensis SLWX₂, Halomonas alkaliphila X₃, and Alteromonas macleodii SLNX₂, which can remove ammonia, nitrite and organic matters in the marine environment were used to strengthen biofilm formation and to purify static waste water, and the best combination was selected according to its removal efficiency of inorganic nitrogen, TN, and CODMn, for the treatment of flowing waste water. The continuous removal of NH₄⁺ -N, NO₂^– -N, and NO₃^– -N from flowing discharged aquaculture waste water was compared with that of naturally formed biofilters. Static waste water treatment results showed that the concentration of NO₃^– -N increased then decreased and that the removal efficiency of inorganic nitrogen and organic matter from the waste water in trial groups was better than that in the control group. Among the treatments, the group of highly concentrated SLWX₂+X₃+SLNX₂ had the best purification effect on all indexes for waste water, and the removal rates of NH₄⁺ -N NO₂^– -N, CODMn, and TN were 100%, 100%, 80.7%, and 59.5%, respectively, after 48 h. In the control group, they were 95.5%, 50.52%, 38.1%, and 13.44%, respectively, and the concentrations of NO₃^– -N kept increasing. The results showed that enhanced biofilm formation by nitrogen-removal bacteria can significantly improve the purification efficiency of biofilters and effectively reduce the concentration of nitrogen and organic matter in waste water from mariculture. The results of the experiment on purification of continuously flowing waste water showed that the concentration of NH₄⁺ -N, NO₂^– -N and NO₃^– -N in the effluent of the experimental groups and the control group was lower than that in the influent. Furthermore, these measurements from the bacteria-augmented biofilm group were lower than those from the natural biofilm and the removal efficiency of inorganic nitrogen in the 10⁶ CFU/mL experimental group was the highest, with the maximum removal rates of 31.6%, 11.33%, and 15.6%, respectively. This was followed by the 10⁵ CFU/mL test group, and the concentration of inorganic nitrogen in the effluent could be maintained at low levels for over 21 days, suggesting that the continuous removal effect of the enhanced biofilm was better than that of the natural biofilm. The results of this study provided a theoretical and technical basis for the application of nitrogen-removing bacteria in the purification of waste water from mariculture.