Myxobolus honghuensis is a parasitic pathogen that causes pharyngeal myxosporidiosis. The infection often results in massive mortality in cultured gibel carp Carassius auratus gibelio (Bloch). Currently, there is no effective drug for treating pharyngeal myxosporidiosis. Most myxosporeans have a complex life history involving two alternative hosts. Myxosporean infection in fish hosts occurs primarily through the horizontal transmission of actinosporean released by invertebrate hosts. Domestic researchers have performed many studies on the life history of myxosporea parasitized by C. auratus gibelio. Approximately 20 species of actinosporean have been discovered and reported. However, no invertebrate host and actinosporean have been found in M. honghuensis. In this study, gibel carp larvae were obtained by artificial insemination, hatched, and nursed in an indoor water circulation system with tap water at a temperature of (23±1) ℃. Single tube semi-nested PCR, fluorescence quantitative PCR, and oligonucleotide fluorescence in situ hybridization (FISH) were used to detect M. honghuensis infection in brood stock tissues, eggs, and larvae. The covet infection rate of M. honghuensis in the 34 gibel carp females used in this study (A1–A22, B1–B12) was 50% to 75%. The positive infection rate in eggs and pseudobranch was higher than that in ovarian tissue samples. The pre-sporogenic stage of M. honghuensis was detected in the ovary, pseudobranch, kidney, and spleen of gibel carp. The 15 and 30 days post-hatch samples of gibel carp larvae (A1, A8, B8, and B9) obtained from infected females and completely cultured indoors were positive for M. honghuensis infection. The presence of M. honghuensis in the pseudobranch, gill, and kidney tissues of 15 days post-hatch larva was confirmed via FISH. The collective results reveal transovarian transmission of M. honghuensis in gibel carp.