Group living is a widespread social phenomenon of animals (e.g., insect swarms, fish shoals, bird flocks, and mammal herds), and collective behavior is affected by ecological contexts and its determination sequence. To investigate the ecological contexts and its determination sequence on the collective behavior of the Cyprinidae fish, the present study used mirror carp (Cyprinus carpio var. specularis) with similar body mass and healthy condition as the experimental fish at (26.3±0.2) ℃. Our study included two treatment groups (i.e., the fixed sequence group and the random sequence group), with each treatment group containing 14 fish shoals of six individuals. The fish stock in each processing group was placed in different ecological contexts (i.e., the open water environment, the food environment, and food plus refuge). We determined the shooting sequence of the random group by drawing lots to simulate environmental changes. Our results showed that the ecological context and determination sequence could reduce the swimming speed and synchronization of Mirror carp individuals. Additionally, the ecological context can increase the distance between the nearest neighbor and the individual. However, the change in the determination sequence reduces the distance between the nearest neighbor in the non-open water environment (the food environment and the food plus shelter environment) of mirror carp. Furthermore, the ecological context and determination sequence could reduce the group swimming speed and percentage time on moving of the group. There was a negative correlation between inter-individual distance and population polarity of the mirror carp. Moreover, the random sequence enhanced this negative correlation between the open water environment and the food plus shelter environment. Our study suggested that ecological context can decrease the coordination and cohesion of fish shoal in the mirror carp. However, the random sequence enhances the coordination and cohesion of fish shoals and reduces the coordination of the open water environment, thus showing the significant cluster behavior of the fish. This positively impacts the information exchange of individual members in the group movement.