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首页 > 过刊浏览>2022年第29卷第12期 >1778-1787. DOI:10.12264/JFSC2021-0236
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光强对多肋藻小孢子体生长及抗氧化生理的影响
作者:
作者简介:

李国梁(1995–),男,硕士研究生,研究方向为藻类资源与利用.E-mail:898587535@qq.com

中图分类号:

S963

基金项目:

国家重点研发计划“蓝色粮仓科技创新”项目(2018YFD0901504)； 财政部和农业农村部国家现代农业产业技术体系项目(CARS-50)； 中国水产科学研究院基本科研业务费“创新团队”项目(2020TD27).


Effects of light intensity on young sporophyte growth and antioxidant physiology of Costaria costata
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    摘要:

    在实验室条件下, 研究了多肋藻(Costaria costata)孢子体在不同光强下的生长、光合作用、营养成分以及抗氧化能力, 以期揭示多肋藻小孢子体对光强的适应性, 为多肋藻海区栽培提供参考。结果发现, 藻体在光照强度 30~120 μmol/(m2 ·s)均具有较快的生长速率, 其中 60 μmol/(m2 ·s)下相对生长速率(RGR)和藻体 PSII 最大光化学量子产量(Fv/Fm)均最高。总光合速率(Pt)和最大表观光合速率(Pnmax)在培养前期(5 d)随光强增加而上升, 随着培养时间的延长(10 d)在 60 μmol/(m2 ·s)下最高; 在 60 μmol/(m2 ·s)下, 藻体呼吸速率(Rd)较低。叶绿素 a (Chl a)、岩藻黄素 (Fucox)和类胡萝卜素(Car)的含量为低光组稍高于高光组(P＞0.05)。粗蛋白、脂肪和粗纤维的含量与光强具有一定的正相关性, 而碳水化合物含量则与光强呈负相关。结果表明, 60 μmol/(m2 ·s)适宜多肋藻小孢子体的生长。在较低或较高光强下, 藻体呼吸作用均显著增强; 此外, 高光下, 可溶性蛋白含量显著上升, 低光下, 抗超氧阴离子自由基(ASAFR)、超氧化物歧化酶 SOD)、抗坏血酸过氧化物酶(APX)比活力较高, 过氧化氢酶(CAT)比活力则在低光和高光下均下降, 这些生理响应对多肋藻小孢子体适应不同光强具有重要的调节作用。

    Abstract:

    Costaria costata is a potential economic kelp. Light intensity is one of the key factors that regulates the growth, photosynthesis, and metabolism of seaweeds, and is also the main factor affecting the morphology and vertical distribution of seaweeds. Therefore, it is important to study the tolerance of C. costata to light intensity for commercial farming. The growth, photosynthesis, nutrient composition, and antioxidant capacity of young sporophytes of C. costata were studied under different light intensities of 30–120 μmol/(m2 ·s) under laboratory conditions to reveal the adaptability of C. costata sporophytes to light intensity and support the cultivation of this species in marine areas. The results showed that the young sporophytes grew fast at 30–120 μmol/(m2 ·s), and both the relative growth rate (RGR) and Fv/Fm were the highest at 60 μmol/(m2 ·s). The total photosynthetic rate and the maximum apparent photosynthetic rate increased with light intensity at the early stage (5 d), and reached the highest at 60 μmol/(m2 ·s) at the late stage (10 d). The respiration rate (Rd) was significantly lower at 60 μmol/(m2 ·s) than at the other light intensities. The contents of chlorophyll a, fucoxanthin, and carotenoid did not differ significantly among the varying light intensities. The content of crude protein, fat, and crude fiber was positively correlated with light intensity, while the content of carbohydrate was negatively correlated with light intensity. The results indicated that light intensity of 60 μmol/(m2 ·s) was suitable for the growth of young sporophytes of C. costata. Furthermore, the content of soluble proteins increases under high light intensities while the activities of ASAFR, SOD, APX were higher under low light intensities. Moreover, the alga had the same response mechanism, namely enhanced respiration, under either low light or high light stress.

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李国梁,汪文俊,李宝贤,梁洲瑞,鲁晓萍,李晓捷.光强对多肋藻小孢子体生长及抗氧化生理的影响[J].中国水产科学,2022,29(12):1778-1787
LI Guoliang, WANG Wenjun, LI Baoxian, LIANG Zhourui, LU Xiaoping, LI Xiaojie. Effects of light intensity on young sporophyte growth and antioxidant physiology of Costaria costata[J]. Journal of Fishery Sciences of China,2022,29(12):1778-1787

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