感染“褐色沉积症”虾夷扇贝的外套膜转录组测序及差异表达基因分析
作者:
作者简介:

于佐安(1982-),男,硕士研究生,研究方向为浅海贝类养殖模式及病害防控.E-mail:abc2033864320@sohu.com

基金项目:

现代农业产业技术体系专项资金项目(CARS-49); 辽宁省渔业科技计划项目(201826); 大连市重点研发计划项目(2021YF16SN015, 2019J12SN60); 大连市揭榜挂帅科技攻关项目(2021JB11SN035).


Transcriptome sequencing and analysis of differentially expressed genes in the mantle tissues of scallops (Mizuhopecten yessoensis) infected with Brown Deposition Syndrome
Author:
  • YU Zuo’an

    YU Zuo’an

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • LI Dacheng

    LI Dacheng

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • LI Hualin

    LI Hualin

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • LIU Xiangfeng

    LIU Xiangfeng

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • TENG Weiming

    TENG Weiming

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • LIU Zhongying

    LIU Zhongying

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • WANG Qingzhi

    WANG Qingzhi

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • ZHOU Zunchun

    ZHOU Zunchun

    Liaoning Ocean and Fisheries Research Institute ; Key Laboratory of Protection and Utilization of Aquatic GermplasmResources, Ministry of Agriculture and Rural Affairs ; Dalian Key Laboratory of Genetic Resources for MarineShellfish, Dalian 116023 , China
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  • 摘要
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    摘要:

    为分析“褐色沉积症”形成分子机制, 探究筏式养殖虾夷扇贝(Mizuhopecten yessoensis)贝壳内侧褐色沉积症状形成原因, 采用 Illumina 高通量测序平台对感染“褐色沉积症”虾夷扇贝和健康虾夷扇贝外套膜组织进行转录组测序分析。结果显示, 患病和健康扇贝外套膜分别获得 43862251 和 41806737 条 clean reads。经参考基因组比对分析, 患病和健康扇贝外套膜表达基因数量分别为 17835 个和 16816 个, 差异表达基因 208 个, 其中上调基因 170 个, 下调基因 38 个。选取 6 个差异表达基因进行荧光定量 PCR (qRT-PCR)验证, 结果证明转录组测序分析可靠。GO 功能富集发现差异基因主要富集在几丁质代谢、含氨基葡萄糖化合物代谢、几丁质结合和氨基糖代谢等与几丁质合成代谢相关的通路; KEGG 通路富集分析发现差异基因主要参与内质网蛋白质合成、内吞作用、剪接体和谷胱甘肽代谢等途径; 对差异表达倍数显著的前 40 个基因分析发现, 编码类咖啡酰辅酶 A-O-甲基转移酶(caffeoyl-CoA O-methyltransferase-like, CCOAOMT-L)、类 1-氨基环丙烷-1 羧酸合酶(1-aminocyclopropane-1-carboxylate synthaselike protein, ACCS-L)、黄蛋白(protein yellow-like)、热休克蛋白 70 (HSP70)、过氧化物酶成熟因子(DUOXA1)、多烯脂肪酸异构酶(poly enoic fatty acid isomerase, PFI)、Na+ /Cl+ 依赖的甘氨酸转运蛋白(sodium-and chloride-dependent glycine transporter, GlyT1)、类酪氨酸酶(putative tyrosinase-like protein, Putative Tyr-3)、Toll 样受体(protein toll)等与蛋白质醌鞣化、免疫、氧化、神经传递相关的基因与褐色沉积症形成密切相关, 揭示了虾夷扇贝外套膜褐色沉积症形成的内在原因。

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于佐安,李大成,李华琳,刘项峰,滕炜鸣,刘忠颖,王庆志,周遵春.感染“褐色沉积症”虾夷扇贝的外套膜转录组测序及差异表达基因分析[J].中国水产科学,2023,30(3):284-296
YU Zuo’an, LI Dacheng, LI Hualin, LIU Xiangfeng, TENG Weiming, LIU Zhongying, WANG Qingzhi, ZHOU Zunchun. Transcriptome sequencing and analysis of differentially expressed genes in the mantle tissues of scallops (Mizuhopecten yessoensis) infected with Brown Deposition Syndrome[J]. Journal of Fishery Sciences of China,2023,30(3):284-296

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