The breeding population of Eriocheir sinensis “Changdang Lake 1” were composed of two parallel breeding strains (strain A and strain B), which were resistant to high density and large size, respectively. To evaluate the breeding effect and genetic progress of the second generation of Eriocheir sinensis “Changdang Lake 1” in odd year population, juvenile and adult crab culture experiment was performed under the condition of ecological culture in outdoor pond. The experimental period lasted for approximately 20 months. During the experiment, multiple growth-proofing and gonadal developmental anatomies were combined with the final harvest size distribution. Compared with the unselected population, the growth performance, gonadal development, and culture effects of the two selected strains were comprehensively evaluated. The average body weight of the two selected strains in the juvenile and adult crab culture stages was always higher than that of the control group, and it was significantly higher than that of the control group in July and from September to November during the juvenile crab culture stage (P＜0.05). For adult crabs, the average body weight of strain B was significantly higher than that of the control group (P＜0.05) in October; however, the difference in the average body weights of the three groups in the other months were not significant (P＞0.05). For adult crabs, the peak periods of puberty molting in male and female individuals were from July 30th to September 10th, and August 20th to September 30th, respectively. The puberty-molting rate of the control group was slightly higher than that of the two selected strains. For the hepatosomatic index (HSI), the HSI of the males in the control group was significantly higher than that of the two selected strains on September 20th, while the HSI of the females in the two selected strains on November 10th was significantly higher than that of the control group (P＜0.05). The gonadosomatic index (GSI) of the two selected strains was similar to that of the control group, and the GSI of female strain B was significantly lower than that of the control group on November 10th (P＜0.05). For culture performance, the final average body weight and yield of the two selected strains in the juvenile and adult crab culture stages were higher than those of the control group (P＜0.05), and the final average body weight of strain B was slightly higher than that of strain A. The feed conversion rate (FCR) of the two selected strains in the juvenile and adult crab culture stages was lower than that of the control group (P＜0.05), and the FCR of strain A in the adult crab culture stage was significantly lower than that of the control group. The number of large-size juvenile crabs (≥15 g) and adult crabs (male≥200 g, female≥175 g) of the two selected strains was higher than that of the control group, while the number of small-size juvenile crabs (≤9 g) was lower than that of the control group. In summary, Eriocheir sinensis “Changdang Lake 1” strain A has the advantages of fast growth, high unit yield, and low FCR, while Eriocheir sinensis “Changdang Lake 1” strain B has the advantages of large average body weight, high proportion of large size crabs and late puberty molting. These two breeding strains have the potential for further breeding.