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17β-雌二醇和曲洛斯坦对大口黑鲈生长及性腺发育的影响
作者:
作者简介:

张东云(1997-),女,硕士研究生,研究方向为水产动物遗传育种研究.E-mail:3466372623@qq.com

中图分类号:

S917

基金项目:

广东省乡村振兴战略专项(2022SPY00003)； 广州市科技计划项目(2024B03J1300, 202201010211)； 中国水产科学研究院基本科研业务费资助项目(2023TD95)； 国家淡水水产种质资源珠江分库项目(FGRC18537)


Effects of 17β-estradiol and trilostane on the growth and gonadaldevelopment of largemouth bass (Micropterus salmoides)
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    摘要:

    为获得大口黑鲈(Micropterus salmoides)雌性化逆转的最适参数, 建立伪雌鱼性别诱导和全雄苗种创制技术,

    采用15 日龄(days post hatching, dph)且体长为(1.00±0.01) cm 的大口黑鲈幼鱼为实验对象, 投喂拌有17β-雌二醇

    (17β-estradiol, 17β-E2)或曲洛斯坦(trilostane, TR)的人工配合饲料, 3 个实验组饲料分别含有30 mg/kg 17β-E2、

    30 mg/kg TR 和20 mg/kg 17β-E2+10 mg/kg TR (依次命名为E30、TR30 和E20TR10), 对照组饲料不含17β-E2 和TR

    (命名为C), 60 d 后停止投喂外源激素, 分析饲喂17β-E2 和TR 对大口黑鲈生长性能、雌性比例、性腺发育的影响;

    12 月龄时, 采集各实验组伪雌鱼和对照组雌雄鱼血液和性腺组织, 检测性类固醇类激素含量、dmrt1 和cyp19a1a

    基因表达水平及卵巢发育形态。结果表明, 饲喂外源雌性激素60 d 后, E30 和E20TR10 组的体长和体重显著低于C

    组(P<0.05), TR30 组的体长和体重高于对照组但无显著差异(P>0.05), E30、TR30 和E20TR10 组雌性比例分别为

    100.00%、0%、100.00%, C 组雌性比例为53.33%; 性腺组织切片结果显示, C 组卵巢有大量初级卵母细胞, E30 和

    E20TR10 组遗传雄鱼出现卵巢腔、卵原细胞和卵母细胞, 而TR30 组雄鱼未发生性逆转; 12 月龄时, 各实验组伪雌

    鱼比例未发生变化, C 组雌性比例为46.67%; E30 和E20TR10 组伪雌鱼的卵巢发育迟滞, 处于II 期; 此外, E30 和

    E20TR10 组伪雌鱼血清中雌二醇(estradilol, E2)含量显著高于对照组雄鱼(P<0.05), TR30 组低于对照组雄鱼, 但无

    显著差异(P>0.05), 各实验组血清中睾酮(testosterone, T)含量显著高于对照组雌鱼(P<0.05)。与对照组雄鱼相比,

    E30 和E20TR10 组伪雌鱼cyp19a1a 表达量显著上调(P<0.05), dmrt1 表达量显著下调(P<0.05)。综上所述, 本研究

    投喂30 mg/kg TR 未获得伪雌鱼, 而投喂30 mg/kg 17β-E2 和20 mg/kg 17β-E2+10 mg/kg TR 实验组均可获得大口黑

    鲈伪雌鱼。12 月龄伪雌鱼卵巢处于II 期, 血清中E2 含量及卵巢中cyp19a1a 表达量均未到达正常雌鱼水平, 导致

    卵巢发育迟滞。

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张东云,宋红梅,李胜杰,田太行,杜金星,朱涛,雷彩霞,朱俊杰.17β-雌二醇和曲洛斯坦对大口黑鲈生长及性腺发育的影响[J].中国水产科学,2024,31(3):266-277
ZHANG Dongyun, SONG Hongmei, LI Shengjie, TIAN Taihang, DU Jinxing, ZHU Tao, LEI Caixia, ZHU Junjie. Effects of 17β-estradiol and trilostane on the growth and gonadaldevelopment of largemouth bass (Micropterus salmoides)[J]. Journal of Fishery Sciences of China,2024,31(3):266-277

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