为探究福建牡蛎(Crassostrea angulata)在急性低氧胁迫下鳃组织的氧化应激反应和闭壳肌的能量代谢, 本研

究设置6 个溶解氧浓度梯度(0.5 mg/L、1.0 mg/L、1.5 mg/L、2.0 mg/L、3.0 mg/L、4.0 mg/L), 对福建牡蛎实施持续

96 h 低氧胁迫处理。采用酶联免疫分析技术分析低氧胁迫第6、12、24、48、72、96 小时福建牡蛎鳃组织的总抗

氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性和丙二醛(MDA)含量以及闭壳肌组织的碱

性磷酸酶(AKP)活性、乳酸脱氢酶(LDH)活性和糖原含量。结果显示: (1) 6~24 h 的低氧胁迫下, T-AOC、SOD 活性

和MDA 含量呈现先升高后降低的现象, CAT 活性则表现为先降低后升高。胁迫24 h 时全部处理组的MDA 含量和

部分处理组的T-AOC 和SOD 活性恢复至对照组水平。24~96 h 的低氧胁迫下, T-AOC、SOD 活性和MDA 含量呈

现先升高后降低再升高, 部分处理组CAT 活性持续降低。低氧胁迫96 h, 全部处理组的T-AOC 活性和部分处理组

的MDA 含量及CAT 活性恢复至对照组水平; 除1.0 mg/L 浓度组与对照组无显著差异(P>0.05), 其余处理组SOD

活性显著低于对照组(P<0.05)。(2) 在96 h 低氧胁迫过程中, 部分处理组LDH 活性在6~12 h 和24~48 h 期间显著

升高(P<0.05); 各低氧处理组的AKP 活性随胁迫时间增加先降低后升高; 糖原含量随低氧胁迫时间增加发生不同

程度的下降, 当胁迫至96 h 时, 除0.5 mg/L 浓度组的糖原含量显著降低(P<0.05)之外, 其他组糖原含量恢复到与对

照组无显著差异(P>0.05)。上述结果表明, 急性低氧胁迫下, 福建牡蛎通过调整体内不同酶活性变化, 改善机体氧

化应激反应和能量代谢的收支平衡, 但极端的低氧环境胁迫仍会对福建牡蛎造成不可修复的损伤。

To investigate the oxidative stress response of gill tissues and the energy metabolism of the adductor

muscle of Crassostrea angulata under hypoxic stress, six dissolved oxygen concentration gradients (0.5 mg/L,

1.0 mg/L, 1.5 mg/L, 2.0 mg/L, 3.0 mg/L, 4. 0 mg/L) were used to treat C. angulata under hypoxic stress for 96 h.

The total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, catalase (CAT) activity,

malondialdehyde (MDA) content of C. angulata gill tissues, alkaline phosphatase (AKP) activity, lactate

dehydrogenase (LDH) activity, and glycogen content of the adductor muscle were determined using enzyme-linked

immunosorbent technique at the 6th, 12th, 24th, 48th, 72nd, and 96th hour of hypoxic stress. The results showed

that: (1) under low oxygen stress for 6–24 h, T-AOC, SOD activity, and MDA content showed the phenomenon of

first increasing and then decreasing, while CAT activity showed the phenomenon of first decreasing and then

increasing; at 24 h, the MDA content of the whole treatment group and the T-AOC activity and SOD activity of

part of the treatment group were restored to the level of the control group; and under hypoxic stress for 24–96 h,

T-AOC, SOD activity, and MDA content increased, then decreased, and then increased again, and CAT activity of

some treatment groups continued to decrease. T-AOC activity of all treatment groups and MDA content and CAT

activity of some treatment groups recovered to the level of the control group at 96 h, except for the 1.0 mg/L

concentration group, which did not have any significant difference from the control group. The SOD activity of the

rest of the treatment groups was significantly lower than that of the control group (P<0.05). (2) Over the 96 h of

hypoxic stress, LDH activity significantly varied among different treatment groups, with a total of three peaks

(P<0.05). AKP activity initially decreased and then increased with stress duration. Glycogen content decreased to

varying degrees with prolonged hypoxic stress time, and after 96 h, the 0.5 mg/L concentration group, showed a

significant decrease in glycogen content (P<0.05), while the other groups showed no significant differences from

the control group. These results suggest that C. angulata exhibits strong hypoxia tolerance by modulating changes

in various enzyme activities within the body to enhance the balance of body's oxidative stress response and energy

metabolism. However, extreme hypoxic environmental stresses still affect the physiological state of C. angulate.