花鲈对不同光色的趋向行为与生理适应特性
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中国水产科学研究院黄海水产研究所/深蓝渔业工程联合实验室 山东 青岛 266071

作者简介:

马斌(1999-),男,硕士学历,研究方向为海水鱼类行为与生理学.E-mail:1006659445@qq.com

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S917

基金项目:

国家海水鱼产业技术体系项目(CARS?47); 山东省重点研发计划项目(2023TZXD050); 青岛海洋科技中心山东省专项经费项目(2022QNLM030001); 中国水产科学研究院基本科研业务费项目(2023TD51; 2024XT0701).


Behavioral and physiological adaptation characteristics of Lateolabrax maculatus to different light color
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Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Joint Laboratory for Deep Blue Fishery Engineering, Qingdao 266071 , China

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    摘要:

    为研究鱼类对不同光色的趋向行为与生理适应特性, 本研究通过光色趋性装置观测了花鲈(Lateolabrax maculatus)幼鱼个体和群体的光色趋向行为, 并分析了其在偏好光色下肝脏酶活力、视网膜组织形态以及眼组织的转录特性。分别在红、黄、蓝、绿和白5个光色区域下, 观测了花鲈幼鱼个体和群体的趋色行为, 发现其在蓝光和白光下群体趋色指数(GPI)和停留时间(GRT)均显著高于其他3种光色(P<0.05)。但其群体和个体对光色偏好存在差异, 群体对蓝光的趋向性更强, 而个体更偏好在白光区域停留。此外, 花鲈在红光和黄光区域保持较高频次的摆尾频率(GTBF)。从生理适应特性角度分析, 与绿光组相比, 蓝光组花鲈幼鱼肝脏的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)的活性以及丙二醛(MDA)含量显著较低(P<0.05)。与绿光组和白光组相比, 蓝光组花鲈幼鱼的视网膜视锥细胞数量具有增多的趋势, 但其色素指数 (PI)偏低。眼球转录组的KEGG注释结果表明, 与白光组相比, 绿光组差异表达基因(DEGs)主要富集在钙信号通路 (calcium signaling pathway), 其中通路内 α7nAChR、casq 等基因上调表达。此外, 与蓝光组和白光组相比, 绿光上调了花鲈眼球中马达蛋白(motor proteins)信号通路中的myh、tnn2、tpm1等基因的表达。综上, 与其他4种光色相比较, 花鲈对蓝光的趋向更强, 且位于蓝光下所受的应激程度较低。

    Abstract:

    This study investigated the behavioral and physiological adaptation characteristics of Lateolabrax maculatus to different light colors at both population and individual levels. We analyzed the liver enzyme activity, retinal tissue morphology, and eye transcriptomics under preferred light colors. Five light colors (red, yellow, blue, green, and white) were used to explore population and individual color peference behavior. The results showed that the population color trend index (PCTI) and population retention time (PRT) of Lateolabrax maculatus were significantly higher under blue and white lights than those of other (P<0.05). However, differences were observed in light-color preferences between populations and individuals; populations showed a stronger preference for blue light, whereas individuals preferred white light. Additionally, higher tail beat frequency (PTBF) was observed in red and yellow light regions. Physiological analysis indicated that hepatic antioxidant parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), alkaline phosphatase (AKP), acid phosphatase (ACP), and malondialdehyde (MDA) content were significantly lower (P<0.05) under blue light than those under green light. Retinal analysis showed an increased number of cone cells (C. C) under blue light compared to green and white light, though the pigment index (PI) was lower. The KEGG annotation of the eye transcriptome showed that differentially expressed genes (DEGs) in the green light group were mainly enriched in the calcium signaling pathway compared with that in the white light group, in which genes such as α7nAChR and casq were up-regulated within the pathway. Furthermore, green light upregulated motor protein signaling pathway genes (myh, tnn2, and tpm1) in the eyes of periwinkles compared to the blue and white light. In summary, Lateolabrax maculatus populations displayed a stronger tendency toward blue light and experienced lower stress levels under blue light compared to other light colors.

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马斌,徐永江,姜燕,郑纪昌,焦凯丽,刘航,李腾坤.花鲈对不同光色的趋向行为与生理适应特性[J].中国水产科学,2024,31(10):1204-1217
MA Bin, XU Yongjiang, JIANG Yan, ZHENG Jichang, JIAO Kaili, LIU Hang, LI Tengkun. Behavioral and physiological adaptation characteristics of Lateolabrax maculatus to different light color[J]. Journal of Fishery Sciences of China,2024,31(10):1204-1217

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  • 收稿日期:2024-10-08
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  • 在线发布日期: 2025-01-02
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