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秦皇岛昌黎海域微生物群落与抗生素耐药基因季节性分布特征
作者:
作者单位:

1.北京市科学技术研究院分析测试研究所(北京市理化分析测试中心), 北京 100089 ;2.国家卫生健康委员会食品安全风险评估重点实验室, 北京 100022 ;3.中国水产科学研究院, 农业农村部水产品质量安全控制重点实验室, 北京 100141 ;4.北京市计算中心, 北京 100094

作者简介:

王秋水(1991-),女,博士,副研究员,研究方向为微生物多样性分析及抗生素抗性基因赋存表征.E-mail:wangqius9182901@hotmail.com

中图分类号:

S917

基金项目:

国家卫生健康委食品安全风险评估重点实验室开放课题资助课题项目(2024-15)； 世界自然基金会蔚蓝星球基金三期项目(PORO001427)


Seasonal distributions of microbial communities and antibiotic resistance genes in the coastal waters of Changli County, Qinhuangdao City
Author:
Affiliation:

1.Institute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical andChemical Analysis), Beijing 100089 , China ;2.NHC Key Laboratory of Food Safety Risk Assessment, Beijing 100022 , China ;3.Key Laboratory of Aquatic Product Quality and Safety Control, Ministry of Agriculture and Rural Affairs, ChineseAcademy of Fishery Sciences, Beijing 100141 , China ;4.Beijing Computing Center, Beijing 100094 , China

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    摘要:

    为深入了解秦皇岛昌黎海域微生物群落结构与抗性基因季节性赋存特征, 采用宏基因组测序技术对昌黎海域的海水、底泥和矛尾复虾虎鱼(Synchogobius hasta)肠道内容物进行分析。结果显示, 昌黎海域样本微生物组成主要是细菌类, 最优势的细菌门是变形菌门(Proteobacteria), 最优势的细菌属是发光杆菌属(Photobacterium)。海水中的主要抗生素耐药基因(antibiotic resistance genes, ARGs)类型是 APH2_IIa、vanXYG、OXA_325 和 TriC, 底泥中的最主要抗生素耐药基因类型是 bcrB、vanHO、adeS 和 EreA 等, 矛尾复虾虎鱼肠道内容物中抗生素耐药基因主要是 msrE、emrK 和 QnrB4 等。网络分析揭示了该海域的属水平的微生物类群与抗生素耐药基因高度相关, 并且主要类群的属都是多重耐药菌株, 抗生素的外排泵是该微生物群落主要的抗生素耐药机制。本研究发现昌黎扇贝养殖区具有丰富的抗生素耐药菌和抗生素耐药基因, 且与水产养殖生产中常用抗生素类别较为吻合, 提示后续应加强对该养殖海域中抗生素耐药菌和抗生素耐药基因的持续监控。

    Abstract:

    The extensive use of antibiotics in industrial and agricultural activities has resulted in environmental deterioration, drug residues, bacterial resistance, and imbalanced microbial structure in the marine environment, affecting the function of the marine ecosystem. To understand the seasonal distributions of microbial communities and resistance genes in the sea area of Changli County, Qinhuangdao City, metagenomic sequencing technology was used to sample and analyze the seawater, sediments, and intestinal contents of the local fish, Synechogobius hasta. The microbial composition of the seawater samples in Changli County was mainly bacterial. Proteobacteria was the most dominant bacterial phyla, with Photobacterium being the most abundant genus. The main types of antibiotic resistance genes in seawater were APH2_IIa, vanXYG, OXA_325, and TriC, whereas in sediments were bcrB, vanHO, adeS, and EreA. Meanwhile, the main types of antibiotic resistance genes in the intestinal contents of the Synechogobius hasta were msrE, emrK, and QnrB4. Network analysis revealed that microbial genus level in the sea area was highly associated with antibiotic resistance genes, and the main genera were multi-resistant strains. The efflux pump of antibiotics is the main antibiotic resistance mechanism in this microbial community. The scallop farming area in Changli County is rich in antibiotic resistant bacteria and genes, which align with the commonly used antibiotic categories in aquaculture production. Enhancing the continuous monitoring of antibiotic-resistant bacteria and genes in the aquaculture area is urgently required.

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王秋水,邓婕,王胜楠,程波,刘悦,袁寒玉,王伟,高丽娟.秦皇岛昌黎海域微生物群落与抗生素耐药基因季节性分布特征[J].中国水产科学,2024,31(11):1399-1410
WANG Qiushui, DENG Jie, WANG Shengnan, CHENG Bo, LIU Yue, YUAN Hanyu, WANG Wei, GAO Lijuan. Seasonal distributions of microbial communities and antibiotic resistance genes in the coastal waters of Changli County, Qinhuangdao City[J]. Journal of Fishery Sciences of China,2024,31(11):1399-1410

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  • 收稿日期:2024-06-04
  • 最后修改日期:2024-08-23
  • 在线发布日期: 2025-02-11
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