To establish the method for removing high-abundance proteins in shrimp plasma proteomics analysis，depletion of plasma high-abundance proteins，such as hemocyanins，was practiced by ultracentrifugation. The efficiency of the method was assessed by 2-DE. The ultracentrifuge method for removing plasma high-abundance proteins was established to remove most hemocyanins. The proteins spots of 2-DE map were more clear and some low-abundance proteins were visualization after ultracentrifugation. The established methods for high-abundance depletion can be effectively applied in shrimp and other crustaceans plasma proteomic study.