鲤的微卫星标记与体质量、体长、体高和吻长的相关分析
DOI:
CSTR:
作者:
作者单位:

1. 中国水产科学研究院 黑龙江水产研究所及农业部北方鱼类生物工程育种重点开放实验室,黑龙江 哈尔滨 150070;2.大连水产学院 生命科学与技术学院,辽宁 大连 116023

作者简介:

通讯作者:

中图分类号:

基金项目:


Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    利用24个鲤(Cyprinus carpio L.)EST-SSRs标记和41个SSRs标记对柏氏鲤和荷包红鲤抗寒品系回交子代的84个个体基因组DNA进行检测,共得到182个等位基因,各座位等位基因数2~4个,片段长度142~329 bp,有效等位基因数1.391 3~3.935 3,观察杂合度0.321 4~1.000 0,期望杂合度0.282 9~0.753 5,平均位点多态信息含量0.512 0。利用统计软件SPSS的GLM程序对65个微卫星标记与鲤鱼体质量、体长、体高和吻长的相关性进行了分析,其中与体质量、体长显著相关的标记是HLJE365;与体质量、体高显著相关的标记是HLJ816;与体质量显著相关的标记是HLJ726,与体长显著相关的标记是HLJ107HLJ1098;与体高显著相关的标记是HLJ111HLJ817;与吻长显著相关的标记是HLJE331、 HLJ906、 HLJ1330。对同一标记不同基因型间进行多重比较,找到与4种性状相关的基因型,并运用数学建模的思想定位等位基因对性状的影响。

    Abstract:

    Sixty-five microsatellite markers(24 EST-SSRs markers and 41 SSR markers) were selected to analyze the genomic DNA of 84 progenies derived from the recombinant backcross lines (BC) of common carp,which came from the backcross between Barbless carp and Hebao-cold tolerance red carp. The results showed that a total of 182 different alleles were found,and the number of alleles in each locuswas 2 to 4. The DNA fragment length was 142 bp to 329 bp,and the number of valid alleles was 1.391 3 to 3.935 31. The value of observed heterozygosity was 0.321 4 to 1.000 0,expected heterozygosity was 0.282 9 to 0.753 5 and the mean polymorphism information content (PIC) was 0.512 0. A GLM procedure was used to analyze the correlation between the 65 microsatellites and body weight,length height and upper jaw length wensize. Results uncovered that HLJ852 had a significant impact on body weight,length and Upper jaw length wensize,and HLJ365 had a significant impact on body weight and height. HLJ816 had a significant impact on body weight and body height. HLJ726 had a significant impact body weight. HLJ107 and HLJ1098 had a significant impact on body length. HLJ111 and HLJ817 had a significant impact on body height. HLJE331,HLJ906 and HLJ330 had a significant impact on upper jaw length wensize. In addition,the genotypes of these correlative loci were investigated with the aid of mathematical modeling idea.

    参考文献
    相似文献
    引证文献
引用本文

杨晶,张晓峰,储志远,孙效文. 鲤的微卫星标记与体质量、体长、体高和吻长的相关分析[J].中国水产科学,2010,17(4):619-629
.[J]. Journal of Fishery Sciences of China,2010,17(4):619-629

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2010-08-11
  • 出版日期:
文章二维码