Amplified fragment length polymorphism (AFLP) was used to assess the genetic diversity of five wild populations of from Dalian province which were Shichengdao(SCD), Jiangjunshi(JJS), Dachangshan(DCS), Dongtuozi(DTZ), Yanchang(YC) and a Shandong population which was Penglai(PL). Five hundred and thirty loci were obtained with ten combinations of selective primers. Of these loci, 417 were polymorphic, accounting for 78.98% of the total. Analysis of molecular variance (AMOVA) revealed that a higher portion (76.58%) of variations existed within populations, while lower portion (23.42%) existed among populations. The (0.234 2), indicating great genetic differentiation within populations. UPGMA dendrogram of the six populations showed they could be clustered into two major groups. One group was from , and the other was from Dalian province. Among the populations from Dalian province, JJS and DCS were clustered into one group, and the coeflarity between them is 0.769 8; but YC was lonely clustered into one group, whose coefficients of similarity with other populations were below 0.583 0. The results showed that there was higher genetic diversity within the six populations, but lower diversity among the populations, and genetic distance of populations was decided not only by geographical distance but also by externalhe great genetic differentiation among populations may result from different reproduction forms, little gene flow or different growth environments