In this study, closed population continuing selection and breeding method was carried out on the selective breeding of Nile tilapia)with main selective characters such as growth rate, stripe identity in caudal fin and body shape. And the selection efficiencies of early generations (second generation) were evaluated. The results were as follows: (1) In selection generation (SG) population, the percentage of females suitable for selective breeding in P2 generation of Nile tilapia was 103.16% and 35.92% higher than that in the foundation (P0) and first (P1) generations, and the percentage in P1 generation was 49.47% higher than that in P0 generation, respectively, which were much higher than that in control generation (CG) population which was not selected and bred; (2) Approximately 0.215 million hatchlings were produced by the cross of Nile tilapia in SG population, higher than that in CG population (0.212 million hatchlings). The male rate of hybrids of Nile tilapia(♀) × (♂) in SG population(“Mei-Ao” tilapia)were 96.27%, 97.01% and 95.70% in pre-reproduction, middle-reproduction and post-reproduction stages, respectively, which were higher than that in CG population (94.37%, 95.65% and 94.18%); (3) The average body weight, average body length, absolute growth rate of body weight, absolute growth rate of body length, specific growth rate of body weight and growth index in SG population after different culturing duration were higher than those in CG population. The percentage of fish with above 600 gram size was 64.6% in “Mei-Ao” tilapias generated by SG population(test group), which was 26.2% higher than that in “Mei-Ao” tilapias generated by CG population(control group). The growth rate of test group was much higher than that of control group. The percentage of fish with head length/body length ratio under 0.30 was 32.07% in test group, which was 68.8% higher than control. The survival rate, male rate, meat rate, average production per m² and relative fatness in each test group of “Mei-Ao” tilapia were higher than those in the corresponding control group, and the feed coefficient was lower than that in control group. These results indicated that the selective effects of Nile tilapia were prominent and enough parental fish could be offered for crossbreeding of