This study was conducted to evaluate the effects of dietary lipid sources on growth, digestive enzyme activity and fatty acid composition in juvenile red claw crayfish, . Four oils, peanut oil, pork lard, fish oil and soybean oil, were added to the based diets containing 5% respectively. After 8 weeks, there were no significant difference (0.05) on length growth rate and special growth rate among all treatments. Red claw crayfish fed the diet with soybean oil had significantly (0.05) higher weight gain (2332.93%) and hepatopancreatic index (0.75%). The highest survival rate was found in red claw crayfish fed diet with soybean oil (83.3%), but lowest with fish oil (56.7%). Analysis of digestive enzyme profiles from the hepatopancreas revealed a positive correlation between enzyme activity and the diet lipid sources. There was no significant difference (0.05) among different lipid sources, which was falling as fish oil, contol, soybean oil, pork lard and peanut oil. Cellulase activity was highest (). Fatty acids composition, including Saturated fatty acids (SFA), Mono-unsaturated fatty acids (MUFA) and Poly-unsaturated fatty acids (PUFA) of tail muscle in juvenile red claw crayfish, was affected by different lipid sources. There were significantly highest content of SFA in pork lard group (0.05).Red claw crayfish fed diet with soybean oil had better growth index and enzyme activities compared to other diets. The content of PUFA especially linoleic acid and linolenic acid in tail muscle was higher than other groups. Based on these results, it suggested that using soybean oil as lipid source can afford more essential fatty acids (EFA) to juvenile red claw crayfish, which have better effects on growth, and also reduce the cost.