Occurrence of poisoning caused by tetrodotoxin(TTX) are frequently reported in recent years. The establishment of a good detection method might be helpful to promote the monitoring works of TTX in aquatic product. Impact of extraction method and chromatographic conditions on TTX detection were studiedlow-cost detection method has been developed in this study. Puffer fishes were selected as the main research material. After ultrasonic extracting and WCX solid-phase extraction cartridge in sequencethen separated by reverse phase ion-pair liquid chromatography and detected by UV-detector. The recovery rates were 75%–82%. Relative standard deviations (RSDs) were less than 15%，sensitivity and operability of this method is better than other detection methods, whose application would be pushed forward more easily .