水产品中河鲀毒素的高效液相紫外测定法
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中国水产科学研究院 南海水产研究所,广东 广州 510300

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    摘要:

    联用固相萃取纯化,使用高效液相色谱紫外检测方法检测水产品中河鲀毒素含量,探索提取方法和色谱条件对检测的影响,并确定和优化色谱分离和紫外检测条件,建立了一种高效液相紫外检测法测定水产品中河鲀毒素的方法。该检测方法稳定性好,可操作性强,加标回收率在之间,相对标准偏差小于

    Abstract:

    Occurrence of poisoning caused by tetrodotoxin(TTX) are frequently reported in recent years. The establishment of a good detection method might be helpful to promote the monitoring works of TTX in aquatic product. Impact of extraction method and chromatographic conditions on TTX detection were studiedlow-cost detection method has been developed in this study. Puffer fishes were selected as the main research material. After ultrasonic extracting and WCX solid-phase extraction cartridge in sequencethen separated by reverse phase ion-pair liquid chromatography and detected by UV-detector. The recovery rates were 75%–82%. Relative standard deviations (RSDs) were less than 15%,sensitivity and operability of this method is better than other detection methods, whose application would be pushed forward more easily .

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岑剑伟,李来好,杨贤庆,魏涯,刁石强,辛少平,周婉君,石红.水产品中河鲀毒素的高效液相紫外测定法[J].中国水产科学,2010,17(5):1036-1044
.[J]. Journal of Fishery Sciences of China,2010,17(5):1036-1044

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  • 在线发布日期: 2010-10-14
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