was reported to be a new marine pathogen that had caused vibriosis of fishes. In Qinhuangdao, Hebei Province was one of the causative agents of vibriosis that endangered the aquaculture of Japanese flounder , and no effective therapy was found other than antibiotics. Vaccines have been successfully used to control vibriosis in aquaculture, and information on the outer membrane proteins (OMPs) of several other pathogenic vibrio species have been disclosed. However, little is known about this pathogen. Outer membrane proteins (OMPs) are highly immunogenic bacterial components due to their exposed epitopes on cell surface. Interests have been attracted to understand the biological function of OMPs and their immunogencity characteristics in immune response. The present study was initiated to analysis the antigenicity of OMPs instrain HQ010712-1 and compared with three universal marine vibriosis species such as , so as to throw some insight to the protective antigens of the bacteria and facilitate vaccine development. were obtained by Sarkosyl method combining super centrifugal purification. SDS-PAGE showed that the strain HQ010712-1had 8 membrane proteins with molecular weights of 102 kD, 45 kD, 39kD, 36 kD, 30 kD, 28 kD, 24 kD and 22 kD. OMPs of the 4 strains of were analyzed and compared by using SDS-PAGE. The results showed that molecular weights focused on 22 with rabbit antiserum against strain HQ010712-1 whole cells, only 2 OMPs of strain HQ010712-1with molecular weights at 45 kD and 36 kD were detected. It showed that the 2 OMPs had some antigenicity. And the other 3 strains of all had positive reaction. The band of 36 kD had visible positive reaction in the .