The present paper reported the effects pre-operative conditions, ages and nucleus size on pearl performance of pearl oyster . It was found that there were not significant differences in survival rate among the four groups ( > 0.05), which were treated by gamete production induced by temperature and air-dried in the shade, gonad inhibited and non-treatmentin the pearl culture. There were significant differences in the nucleus retention, pearl obtained and high quality pearl rates among the four groups treated by pre-operative conditions ( < 0.05). The groups treated by gretention, pearl obtained and high quality pearl rates than the other three groups, while non-treated groupretention, pearl obtained and high quality pearl rates. Differences in survival rate during the period of recuperation and culture among 1.5, 2.0, 2.5 and 3.0 year old groups were significant ( < 0.05). The survival rates during the period recuperation and culture were the highest in the 1.5 year old group and the lowest in the 3.0 year old group, respectively. The retention and pearl obtained rates were the highest in the 2.0 year old group, while the values were the lowest in the 3.0 year old group.survival rate during the period recuperation and culture among the four groups (< 0.05), where the 5.5, 6.0, 6.5 and 6.0mm size nucleus with were individually transplanted. The survival rate during the period recuperation and culture were the highest in the 5.5 mm size nucleus group and the lowest in the 7.0 mm size nucleus group, respectively. The retention and pearl obtained rates were the highest in 6.0 mm size nucleus group, while the values were the lowest in the 7.0 mm size nucleus group. It was concluded that the pre-operative condition is an important approach to improving the survival rate during the period recuperation and culture, . individuals with ages ranging from 1.5 to 2.0 year old were optimal for nucleus transplantation operation. The nucleus size transplanted is 6.0mm for pearl oyster