Natural resistance associated macrophage protein (Nramp) family is a novel family of functional related proteins with innate resistance to intracellular parasites in mammals, which has the capacity of enhancing macrophages that are meant to kill pathogens. However, few reports are available about the structure and function of Nramp gene was amplified from liver of grass carp () by rapid amplification of cDNA ends (RACE). The full-length cDNA of gene was 3 158 bp, including a 155 bp 5′ terminal UTR, a 1 368 bp 3′ terminal UTR and a 1 635 bp open reading frame encoding a protein with 554 amino acid residues contained the signature features of the Nramp protein family: 12 transmembrane (TM) domains, a consensus transport motif (CTM) with 20 amino acid residues between the transmembrane domains 8 and 9. The deduced amino acid sequence of grass carp sequences. the unique feature of grass carp Nramp was the presence of two UTR and 5UTR. Phylogenetic analysis revealed that the grass carp Nramp was clustered with other fish Nramps and was closer to Nramp2 of mammals. transcripts in different tissues indicated that the grass carp transcripts were highly abundant in head kidney and spleen and low in muscle and skin. The grass carp kidney cells (CIK) challenged with the grass carp reovirus (GCRV) could evidently elevate mRNA levels in cell cultures.