福建近海竹荚鱼线粒体DNA控制区和细胞色素b遗传多态性
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厦门大学 海洋与环境学院, 福建 厦门 361005

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牛素芳(1987–), 女, 硕士. 专业方向为海洋生物生化与分子生物学. E-mail: wolf0487@126.com

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福建省科技项目(2007I0005).


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    摘要:

    Trachurus japonicus)的控制区全序列。个变异位点和平均单倍型多样性, 25()。个氨基酸单倍型序列的遗传多样性高于闽东群体。构建的单倍型系统树未出现明显的以地方群体遗传距离(AMOVA), 群体间频繁的基因交流

    Abstract:

    is a pelagic fish in Carangidae family mainly distributing in coastal waters of China, Japan and Korea. It has been an important commercially China since 1960s. But the natural population genetics and resource quantity of have been declined rapidly due to over-fishing, degradation of its natural habitats and so on in recent years, whereas little knowledge is available about its population genetic structure. The distribution of genetic diversity and genetic structure in wild stocks of seems emergent and the conservation strategies should be developed nowadays. The complete mitochondrial DNA control region ( T. japonicus populations collected from coastal waters in Eastern Fujian(=30). The result showed that 66 variable nucleotide sites were detected and 53 haplotypes were identified in two populations for control region sequence, but only three shared haplotypes were observed. The global haplotype diversity ( gene(930 bp), and 25 haplotypes were recovered in 41 specimens. The average haplotype diversity (0.937 gene encoded 330 amino acids and the amino acid chain had only one haplotype. Each population had good haplotypic diversity and low nucleotide diversity in both mtDNA control region and cytochrome one. was chosen as out-group in this study, and neighbor-joining (NJ) phylogenetic tree was constructed based on the haplotypes from the two populations of. The result showed that the two populations might have originated from one clade. Analysis of molecular variance (AMOVA) by ARLEQUIN version 3.1 revealed that the variation occurred mostly within the two populations for no significant population genetic structure existed throughout examined range, which was neighbor-joining phylogenetic tree and haplotype network. The pairwise Kimura 2-paramter genetic distances calculated by MEGA version 4.0 were also small between the was examined by using neutrality tests and mismatch distribution analysis. The neutral test of Fu’ , which is devised specifically to detect population expansion and is sensitive to the presence of singletons in a sample, showed several highly significant negative values and the pairwise nucleotide differences distribution showed a singlet, indicating population expanded had low between the two populations, and this might be caused by population expansion, strongEast China. All these results would be very important and useful for making scientific strategy for the natural resource protection and genetic breeding of this fish.

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牛素芳,苏永全,王军,张丽艳,曾华嵩,张曼.福建近海竹荚鱼线粒体DNA控制区和细胞色素b遗传多态性[J].中国水产科学,2011,18(1):66-74
.[J]. Journal of Fishery Sciences of China,2011,18(1):66-74

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  • 在线发布日期: 2011-01-13
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