Ammodytes personatus and from the waters nearby Soya in Japan were measured.The results indicated that there was some variance between the two species based on the function of body length and body weight and One-way ANOVA results showed that though there was great (70%) overlap in vertebravertebra. The otolith angle was firstly used for identification of the two species. The average otolith angle of [(86.8)otolith angle to identify the two species and