显微介导的远缘基因渐渗技术在鲤育种中的应用
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1. 农业部水产生物技术重点开放实验室,中国水产科学研究院 黑龙江水产研究所, 黑龙江 哈尔滨 150070;2. 哈尔滨师范大学 生命科学与技术学院, 黑龙江 哈尔滨 150025

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闫学春(1964), 男, 副研究员, 主要从事鱼类基因工程育种与分子生物学研究. E-mail: yanxc8@163.com

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国家863计划项目(2009AA10Z105).


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    摘要:

    Fenneropenaeus chinensis), Cyprinus carpio L.)DNAAFLP对显微介导中国明对虾基因鲤进行外源30, , , 片段的显微介导中国明对虾基因鲤的蛋白质含量分别为、而氨基酸的总量分别为、(16.13%), 种鲜味氨基酸含量明显超过对照组均有显著差异丰富鲤的遗传基础

    Abstract:

    Introgressive hybridization is a movement of a gene from one species into the gene pool of another by repeated backcrossing an interspecific hybrid with one of its parent species. It is an important source of genetic variation in natural population. But the natural introgressive does not have the human direct interference while the exotic introgression is induced intentionally and very slowly. Transgenic technique is an extreme example of introgression verified as a successful hybridization from a transgenic plant to a wild relative. Carp is an important species of fish, but has low muscle quality. The study that focused on the feasibility improves the quality of muscle of carp by introgressive hybridization technology. In this constructed by transferring all-fish gene of shrimp to the zygote of common carp ) using mature micro-injection technology. AFLP technology was used to identify the transgenic zygote. The results of genotype of 30 pairs of AFLP markers and analysis of nutrient content of muscle showed that: (1) The gene of shrimp were detected in genome of transgenic carp by 30 pairs of primers of AFLP of shrimp which confirmed that the genomic fragment of shrimp exists in transgenic carp and has no found in contrast ones, then indicates that it should be feasible to transmit genetic material between two distant related species by transgenic method at molecular level. (2) The statistic analysis showed that the contents of crude protein of transgenic carps were 18.37%, 18.47% and 17.99%, which were higher than those in non-transgenic ones(17.06%). Total amino acids contents were f which 4 kinds of amino acids contents in transgenic carp <0.05), such as and alanine. So, the effects of shrimp gene directly transmitted to the carp genome gives rise to increase nutrient content and amino acids. This method would be useful in improving carp muscle quality and heredity base, and was a very powerful technology for DNA transferred between distant related species. In the future, more and more good trait of fish or other species would be improved by this technology, although this technique needs developing further. Transgenic fish will be

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闫学春,张晓峰,刘翠,孙效文,梁利群,曹顶臣,李超,葛彦龙.显微介导的远缘基因渐渗技术在鲤育种中的应用[J].中国水产科学,2011,18(2):275-282
.[J]. Journal of Fishery Sciences of China,2011,18(2):275-282

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  • 在线发布日期: 2011-03-09
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