The study mainly focused on the effects of dietary lipid levels on partial physique indices, the fat deposition and fatty acid profiles of muscle, liver and adipose tissue of peritoneal cavity in the juvenile fish of GIFT (Genetically Improved Farmed Tilapia, ), and investigated its utilization capabilities of the fatty acid in diets. Triplicate groups of fish were fed for 90 days with the experimental diets formulated with increasing lipid levels (lipid levels group of 1.73%, 3.71%, 5.69%, 7.67%, 9.64% and 16.55%) using fish oil as the lipid source. Result showed condition factor of the fish in the 5.69%, 7.67% 9.64% groups at higher values contrasted to other groups. The hepatosomatic index of the 1.73% and 16.55% group were significantly higher than those other groups (<0.05). Fat content of muscle in 7.67% group were significantly higher than 3.71% group (<0.05). It presented an uptrend on fat contents of muscle and liver and a little influence on fat contents of adipose tissue of peritoneal cavity when dietary lipid levels raising. The higher dietary lipid level, the more body fat deposition and the higher n-3 unsaturated fatty acids proportion of tissue total fatty acids. Results indicated that the partial fish physique indices were influenced by dietary lipid levels, especially hepatosomatic index. While the fish was fed an overabundance lipid diet, the fat was easy to be accumulated in the tissues of muscle and liver. The fat contents and fatty acid profiles of fish reflected the dietary lipid level and fatty acid profiles.