1. 集美大学 水产学院, 福建省高校水产科技与食品安全重点实验室, 福建 厦门 361021;2. 厦门大学 海洋与环境学院, 福建 厦门 361005
毛秀秀(1983-), 女, 硕士研究生, 主要从事水产病害研究. E-mail: maoxiu0202@163.com
国家863计划资助项目(2007AA09Z115); 农业部行业专项(nyhyzx07-043-01); 农业部产业技术体系项目(200907 100001); 厦门市科技项目(3502Z20073019, 3502Z20073020).
Aeromonas hydrophila) , , 随着初始菌液浓度的增加25 0.05培养液中添加氯化镁达到生物膜的(), 0.05嗜水气单胞菌其生物膜的形成能力受温度、酸碱度、起始菌液浓度、钙、镁离子浓度等因素的影响肌肉组织提取液也存在能影响生物膜形成的成分。本研究旨在为探讨嗜水气单胞菌生物膜形成机制和致病机理提供参考。
Aeromanas hydrophila B11 was investigated by the method of modified microtiter-plate test. The results showed thatB11 developed stable and evident biofilm on the surface of the polystyrene microtiter plate, and the OD590 value of biofilm reached the secondary peak in 6 h and the highest peak in 22 h and then reduced with incubation time. The OD590 value of the biofilm decreased with densities of initial bacterial suspension. The OD590 value of biofilm recorded at 25 was significantly higher than those recorded at other temperatures) B11 developed evident biofilm under initial pH 510, while no evident biofilm was found under initial pH 34. When 1mmol/L magnesium ion was added, in the biofilm formation of <0.05), while calcium ion significantly promoted the formation of biofilm). Compared with the control group, only biofilm developed on the surface of the microtiter plate coated by the extract of eel’ muscle exhibited remarkably higher value of OD590)microtiter plate coated by other extracts of tissues showed no remarkably difference) B11 strain can form biofilm on the surface of polystyrene microtiter plate; the bacterial biofilm formation can be affected by different environmental factors such as temperature, bacterial concentration, pH, Mg2+ and Ca2+concentrations and so on; muscle extract of eel can promote the biofilm formation.
毛秀秀,覃映雪,邹文政,郭松林,苏永全,鄢庆枇,纪荣兴.致病性嗜水气单胞菌生物膜的形成特性[J].中国水产科学,2011,18(2):371-378
.[J]. Journal of Fishery Sciences of China,2011,18(2):371-378
