After being excised into several cells of fragments, the indoor incubated 2n female gametophytes of matured and produced some juvenile sporophytes without fertilization. Then, the 2n female gametophytes were induced from the vegetative cells of the obtained parthenogenetic 2n juvenile sporophytes, which were removed rhizoids and in a 0.5−1.0 cm length. The parthenogenesis rate of the matured 2n female gametophytes reached 8.4% and 7.4% after 20 days of incubation at 20℃ and 15℃, respectively, which were both higher than 1% at 10℃. The 2n female gametophytes were induced after incubation for 15 days at 20, but for 30 days at 15℃ and for 45 days at 10℃. The induction rate of the 2n female gametophytes was 100% at 20℃, but was 38% at 15℃ and 12% at 10℃ after 60 days of incubation, respectively. The maturation rate of the induced 2n female gametophytes, after being excised into fragments, reached 34% after incubation for 10 days and 100% for 20 days at 20℃, respectively. The 3n juvenile sporophytes were produced by the cross between the induced 2n female gametophyte and the n male gametophyte. In present study a new method of mass production of 2n female gametophytes was founded, which laid a foundation for the scale production of triploid seedlings of .