PCR-RFLP methodology was developed as a tool to assess the incidence of incorrect labeling of sea cucumbers in commercial food products. The technique allows the genetic identification of 16 species of sea cucumber based on PCR amplification of a common 570 bp fragment of the 16S rRNA gene and follows digestion with 3 restriction enzymes (types, respectively. The method was applied to authenticate the exact species of 19 commercial samples, including frozen and dried products. The results showed that 9 samples were incorrectly labeled (47%). Therefore, PCR-RFLP tool really provides convenient, useful and academic approach to identify commercial sea cucumber and makes the traceability to be possible.