利用SSR﹑EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析
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1. 中国水产科学研究院 黑龙江水产研究所, 黑龙江 哈尔滨150070;2. 上海海洋大学 水产与生命学院, 上海 201306;3. 大连水产学院 生命科学与技术学院, 辽宁 大连 116023

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王宣朋(1983), 男, 硕士研究生, 专业方向为水产养殖. Tel: 15045819248; E-mail:pengwx2008@126.com

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国家973计划项目(2010CB126305); 农业部公益性行业科研专项(200903045).


Analysis of feed conversion ratio, bodyweight, body thickness traits of common carp (Cyprinus carpio L.) using SSR, EST-SSR, SNP markers
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1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2. College of Fisheries and Life Science , Shanghai Ocean University, Shanghai 201306, China;3. College of Aqua-life Science and Technology, Dali

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    摘要:

    913336415%。这些标记可作为分子标记辅助育种的参考标记。将得到的与食物转化率显著相关的、HLJE253BLASTHLJE253。将得到的与标记在通过

    Abstract:

    hybrid common carp gynogenetic line including 68 individuals was used as mapping pane1. Markers wNinety-one SSR markers and 33 EST makers and 364 SNP markers were selected to analyze the genomic DNA of 68 progenies derived from common carp full-sib family. Twenty-seven loci were discovered that had significant impact on feed conversion efficiency and thirty-five loci were significantly related with body thickness (<0.05)while twenty seven loci had significant impact on body weight (<0.05). SNP0041 and SNP0044 were related with feed conversion ratio significantly (<0.01), contributing to 15% and 16%, respectively. These markers can be used as molecular marker assisting breeding. Markers were used to screen the protein and nucleotide database in the National Center for Biotechnology Information (NCBI) in order to find the homologies. Blasting results showed that HLJ253 is highly correlated (61%) with the gene encoding structural protein (ORF2) of zebrafish. SNP makers associated with three traits were sequenced on NCBI in order to find related genes and possible functions.

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王宣朋,张晓峰,李文升,张天奇,孙效文.利用SSR﹑EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析[J].中国水产科学,2011,18(3):565-573
WANG Xuanpeng, ZHANG Xiaofeng, LI Wensheng, ZHANG Tianqi, SUN Xiaowen. Analysis of feed conversion ratio, bodyweight, body thickness traits of common carp (Cyprinus carpio L.) using SSR, EST-SSR, SNP markers[J]. Journal of Fishery Sciences of China,2011,18(3):565-573

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  • 在线发布日期: 2011-05-09
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