四引物扩增受阻突变体系PCR技术在中国明对虾SNP基因分型中的研究
作者:
作者单位:

1. 中国海洋大学 海洋生命学院,2. 中国水产科学研究院 黄海水产研究所,3. 山东理工大学 生命科学学院,

作者简介:

张建勇(1977–), 男, 博士研究生, 主要从事水产动物遗传育种研究. E-mail: zhangjy1977@126.com

中图分类号:

S968

基金项目:

国家自然科学基金资助项目(31072206); 山东省自然科学基金项目资助(ZR2009DQ002).


tetra-primer amplification refractory mutation system-polymerase chain reaction in SNP genotyping of shrimp Fenneropenaeus chinensis
Author:
Affiliation:

1. College of Marine Life Sciences, Ocean University of China,2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 3. School of Life Sciences, Shandong University of Technology,

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    摘要:

    及多态性信息含量基因分型的方法。

    Abstract:

    Tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-primer ARMA-PCR) was introduced to investigate single nucleotide polymorphisms (SNPs) genotyping in Chinese shrimp () and 80 were studied. Twenty out of the 80 SNP tetra-primer ARMA-PCR primer sets were validated following touchdown profiles and the outer and the expected inner bands were amplified. Homozygous and heterozygous were detected by agarose gel and the genotypes were obtained. Polymorphism of these distinct loci was assessed using 30 individuals, and the results showed that the 20 loci were all polymorphic. The values of and PIC varied from 1.127 to 1.993, from 0.136 to 0.607, from 0.119 to 0.492 and from 0.145 to 0.3730, respectively. The results indicated that tetra-primer ARMA-PCR is a simple, rapid and efficient method for SNP genotyping which make it useful in a broad aspects of genetic and breeding studies.

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张建勇,王清印,王伟继,孟宪红,孔杰,张全启.四引物扩增受阻突变体系PCR技术在中国明对虾SNP基因分型中的研究[J].中国水产科学,2011,18(4):751-759
ZHANG Jianyong, WANG Qingyin, WANG Weiji, MENG Xianhong, KONG Jie, ZHANG Quanqi. tetra-primer amplification refractory mutation system-polymerase chain reaction in SNP genotyping of shrimp Fenneropenaeus chinensis[J]. Journal of Fishery Sciences of China,2011,18(4):751-759

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  • 在线发布日期: 2011-08-19
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