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斑节对虾C1q结合蛋白(PmC1qBP)的克隆及表达特征分析
作者:
作者单位:

1. 上海海洋大学 水产与生命学院,2. 中国水产科学研究院 南海水产研究所

作者简介:

刘先军(1984-), 男, 硕士研究生, 研究方向: 水产动物遗传育种. E-mail:junxianliua@163.com

中图分类号:

Q959

基金项目:

国家自然科学基金资助项目(40976101); 广东省自然科学基金资助项目(9151030002000008); 农业部948项目(2130106); 物种资源保护项目(2130135); 广东省海洋渔业科技推广专项(A200901 B02).


Cloning and expression profile analysis C1q-binding protein (PmC1qBP) in Penaeus monodon
Author:
Affiliation:

1. College of Fisheries and Life Science, Shanghai Ocean University,2. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,

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    摘要:

    (的胶原样区相互作用并启动多种细胞反应()PmC1qBP基因在性腺、胃、血细胞、肠中表达量存在极显著差异在无节幼体和蚤状幼体时表达量低, 是斑节对虾卵巢发育过程的重要功能基因。应激实验结果表明(Lipopolysaccharide, LPS)PmC1qBP

    Abstract:

    is an important component of the complement system, which interacts with collagen-like region of C1q and invokes many cell responses, involved in enhancing phagocytosis, promoting macrophage of microbial, inducing chemotaxis, and further enhancing the organism's immunity. In recent years, higher frequencies of disease epidemics have been reported. . Now we have successfully constructed cDNA library of ovarian tissue in , and identified PmC1qBP gene. To study the biological functions of PmC1qBP in , we compared the expression level of PmC1qBP in different tissues of female and male and development stages of ovary with semi-quantitative and real-time quantitative PCR. The results show that the expression level of PmC1qBP has significant differences between male and female in gonads, hemocytes, intestine and stomach. At the same time, the expression level of PmC1qBP is very low in nauplii and zoea larvae stage, which increases from mysis larvae stage and stabilizes during the maturation stages of ovary. The results reveal that PmC1qBP is an important functional gene during the ovary development of . The immune challenge experiment indicated that PmC1qBP is up-regulated in hepatopancreas after treated with LPS for 6 h. Penaeus monodon and might play an important function in immune regulation.

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刘先军,杨丽诗,黄建华,周发林,杨其彬,江世贵.斑节对虾C1q结合蛋白(PmC1qBP)的克隆及表达特征分析[J].中国水产科学,2011,18(4):774-781
LIU Xianjun, YANG Lishi, HUANG Jianhua, ZHOU Falin, YANG Qibin, JIANG Shigui . Cloning and expression profile analysis C1q-binding protein (PmC1qBP) in Penaeus monodon[J]. Journal of Fishery Sciences of China,2011,18(4):774-781

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  • 在线发布日期: 2011-08-16
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