1. 辽宁省海洋生物资源恢复与生境修复重点实验室, 大连海洋大学, 辽宁 大连116023; 2. 国家海洋环境监测中心, 辽宁 大连116023; 3. 宝生物工程(大连)有限公司, 辽宁 大连116600;3. 宝生物工程(大连)有限公司, 辽宁 大连116600
杨大佐(1979−), 男, 博士研究生, 助理研究员, 主要从事海洋生物修复技术研究. E-mail: dzyang@dlou.edu.cn
1005−8737− (2011)05−0983−09
国家科技部863项目(2006AA10Z410); 国家海洋公益性行业科研专项经费资助项目(200805069); 国家科技部农业科技成果转化资金项目(2009GB2B000063); 国家自然科学基金项目(30901107).
1. Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China; 2. National Marine Environmental Monitoring Center, Dalian 116023, China; 3. Takara Biotechnology (Dalian), D
表达量均呈显著升高mRNA的应激调控发生于转录水平。本研究为海洋沉积环境早期污染的生态风险预测提供了分子生物学依据。
, by reverse transcription polymerase chain reaction with the primers designed based upon the homologous cDNA sequence of Other primers were designed according to the partial sequence of two fragmentswere obtained using 3RACEand sequenced. Sequence analysis revealed a 904 bp cDNA containing an 81 bp 5untranslated regions, and 357 bp open reading frame putatively encoding a protein of 119 amino acids. The deduced amino acid sequence of MPwas subjected to BLAST search for similarity with published sequences and it was found that MPII shared high identity (81.51%) with Nereis diversicolor (77.12%), and with the hemerythrin of 3EX16HX3HX23/28HX4D) of the hemerythrin familyPerinereis aibuhitensis for 12, 24, 48, and 72 h using quantitative PCR. The expression levels of 2+. The current study showed that the expression levels of the <0.01) than control group at 40 mg/L Cd2+ after 72 h. The level of mRNA produced in the muscle tissue of sandworm increased significantly following exposure to 5, 10, and 20 mg/L <0.05). Therefore, the expression level of the 2+. We suggest that heavy metal Cd2+ is associated with the induction of expression, and the present study provided molecular support for the assessment of early ecological risks for monitoring the marine sediment environment based on the health of clamworm.
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杨大佐,周一兵,陈雪,周笑孝,王斌,袁秀堂,孙静波.双齿围沙蚕金属结合蛋白(MPⅡ)基因克隆与表达[J].中国水产科学,2011,18(5):983-991
YANG Dazuo, ZHOU Yibing, CHEN Xue, ZHOU Xiaoxiao, WANG Bin, YUAN Xiutang, SUN Jingbo. Cloning and expression of the metalloprotein (MPII) gene from polychaetes Perinereis aibuhitensis (Annelida: Polychaeta)[J]. Journal of Fishery Sciences of China,2011,18(5):983-991
