大连海洋大学 农业部海洋水产增养殖学重点开放实验室, 辽宁 大连 116023
李华(1958-), 女, 教授, 研究方向为水产动物病害学．E-mail:lihua@dlou.edu.cn
S94
国家自然科学基金项目(30800853); 国家“十二五”科技支撑计划项目(2011BAD13B03); 海洋公益性行业科研专项(200905020).
Dalian Ocean University, Key Laboratory of Mariculture, Ministry of Agriculture, Dalian 116023, China
研究了培养材料、培养时间、培养基、葡萄糖浓度对菌株生物被膜形成能力的影响并研究了菌株的疏水性。结果表明为高疏水性和高生物被膜形成菌株0.5%型菌毛介导的颤搐等运动力。对抗生素的抵抗力明显强于浮游细菌是浮游细菌的菌株,
infects a range of hosts, including fish, shellfish, and echinoderms. Infection with this pathogen has led to significant economic loss in several cultured species, including the sea cucumber (). Thus, there is an urgent need to understand the pathogenesis of . The virulence of a pathogen is partly a function of its adhesion properties. Adhesion depends on cell surface hydrophobicity and biofilm formation. The hydrophobic cell surface provides an advantage to the bacteria by increasing resistance to phagocytosis or by favoring colonization of mucosal or connective tissue in wounds and endocarditis in bacteria. We evaluated the hydrophobicity and biofilm formation in . We also evaluated the effect of culture constituents, culture time, growth medium, and the concentration of glucose on biofilm formation. We compared the sensitivity of planktonic cells and resuspended AP622 biofilm cells to gentamycin sulphate. Last, we quantified the motility of the flagellum and measured pill and cell surface hydrophobicity using tests for salt-aggregation (SAT) and microbial adherence to hydrocarbons (MATH). AP622 possesses a high ability to form a biofilm. The biofilm formation cycle was 24 h in length and the optimal formation conditions consisted of polyvinyl chloride (PVC) and LB medium with 0.5% glucose. Swarming and twitching motilities played an important role in biofilm formation. The resistance of resuspended biofilm bacteria to antibiotics was significantly higher than intimes higher than in planktonic bacteria. exhibiting strong hydrophobicity, could agglutinate at a concentration of 0.6 mol/L ammonium persulfate, and the adhesion partition to liquid hydrocarbons was >50%. In conclusion, the hydrophobicity and biofilm formation properties suggest that AP622 has strong adhesion properties. Resistance to antibiotics as a result of biofilm formation will reduce the effectiveness of treatments in aquaculture facilities. Thus, cell surface hydrophobicity and biofilm formation play an important role in the pathogenesis of .
李华,王扬,李强,乔帼.灿烂弧菌的疏水性和生物被膜形成能力[J].中国水产科学,2011,18(5):1084-1091
LI Hua, WANG Yang, LI Qiang, QIAO Guo. Cell surface hydrophobicity and biofilm formation ability of Vibrio splendidus[J]. Journal of Fishery Sciences of China,2011,18(5):1084-1091
