海洋生物遗传育种教育部重点实验室, 中国海洋大学, 山东 青岛 266003
王航宁(1983−), 女, 硕士研究生, 主要从事海洋动物生殖生物学研究. E-mail: runningbit@yahoo.com.cn
S917
国家自然科学基金面上项目 (40776074).
Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China,
两端通过肌束分别与呼吸肠管壁外侧和体壁内侧相连。精巢由生精细胞团与结缔组织组成的精巢年周期发育划分为增殖期、生长期、成熟期、排放期和休止期包括细胞核形态的变化、顶体发生以及尾部的形成。本研究揭示了单环刺
We documented the histological structure and annual development of the testis and the process of spermatogenesis in using light microscopy and transmission electron microscopy. The testis was localized at the end of the body and formed a band. Each end of the testis was connected to the body wall and connective tissue and spermatogenic cells, including spermatogonia clusters at the periphery of the connective tissue and interior spermatocytes surrounded by connective tissue. Spermatocytes that were clustered at different stages were transported into the coelomic fluid and developed into sperm, which left the cell clusters and entered the nephridia. Based on the histological characteristics of the testis, we divided the annual development into 5 stages: mature, release, and restingstages. In addition, we observed spermatogenesis in the testis and coelom using both optical and electron microscopy and documented ultrastructural changes in the nucleolus, acrosome, and sperm tail.
[1] 李凤鲁王玮周红我国黄渤海螠虫动物螠虫动物门的研 [J] . 青岛海洋大学学报, 1994, 24 (2) : 203–210．
[2] 李诺宋淑莲唐永政等山东沿海单环刺螠虫繁殖生物学的研 [J] . 动物学报, 1997, 43 (4) : 433–435.
[3] 康庆浩郑家生金在敏单环刺螠的人工苗种生产研究水温对胚胎发育及幼体培育的影响青岛海洋大学学报, 2002, 32 (2) : 273–278.
Ohkawa Y M, Ishida J. Electron microscopical studies on the egg of the gephyrean Urechis unicinctus [J]. Bull Mar Biol Sta Asamush, 1973, 11 : 191–192.
Sawada N, Noda Y. An electron microscope study on the Urechis egg [J] . Mem Ehim Univ Sect II Ser B, 1969, 4 : 551–561.
Meredith G S. Oocyte differentiation in Urechis caupo (Echiura): A Fine structural study[J]. J Morphol, 1975, 147 : 475–506.
[7] 牛从从张志峰邵明瑜单环刺螠虫精子生物学特性和环境因子的关系中国水产科学, 2005, 12(5): 556– 561.
[8] 郑燕玲冯丹青郭丰等星虫动物繁殖生物学研究进展海洋科学, 2006, 30 (1) : 84–87.
[9] 竺俊全王武许式见等可口革囊星虫的精子发生及精子结构动物学报, 2007, 53 (4): 733–741.
Luecht J, Pfannenstiel H D. Spermatogenesis in Platynereis massiliensis (Polychaeta: Nereidae) [J] . Helgolander Mar Res, 1989, 43 (1) : 19–28.
[11] 张红卫发育生物学北京高等教育出版社
[12] 陈大元受精生物学北京科学出版社
Rao X, Chen Y, Chen W, et al. Ultrastructural studies on spermatogenesis of Novaculinn chinensis [J] . Chin J Zool, 2000 , 35 (5) : 2–5.
Z V, Lupetti P, Mencarelli C, et al. Sperm ultrastructure and spermiogenesis of Coniopterygidae (Neuroptera, Insecta) [J] . Arthrop Struct Dev, 2008, 37: 410–417.
王航宁,邵明瑜,张志峰.单环刺螠精巢年周期发育及精子发生[J].中国水产科学,2011,18(5):1189-1195
WANG Hangning, SHAO Mingyu, ZHANG Zhifeng. Annual development of testis and spermatogenesis in Urechis unicinctus[J]. Journal of Fishery Sciences of China,2011,18(5):1189-1195
