悬沙胁迫下熊本牡蛎的损伤及恢复
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中国水产科学研究院 东海水产研究所, 上海 200090

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李云(1979), 男, 博士, 主要从事渔业生态环境研究. E-mail: salixly@yahoo.com.cn

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国家重点基础研究发展规划项目(2010CB429005);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目(2008M13)


Damage and recovery in the Kumamoto oyster Crassostrea sikamea stressed by suspended solids
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East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200090,China

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    摘要:

    -ATPSOD鳃细胞RNA/DNA本研究所设悬沙浓度对牡蛎存活率几乎无影响500 mg/活性开始显著降低RNA/DNA。这些受损牡蛎经干净海水培养检测的生理生化指标虽有, 但分子细胞水平上的各指标就能比较灵敏地反映悬沙胁迫的影响。

    Abstract:

    We evaluated the effects of suspended solids on the survival and physiology of the Kumamoto oyster, . The oysters were exposed to 0, 50, 100, 500, 1 000, or 5 000 suspended solids (SS) for 15 d then returned to clean seawater, without SS, for 15 d. We monitored survival and measured -ATPase, SOD, CAT, and DNA damage in the gills and the RNA/DNA ratio in the muscle tissue+-ATPase, were significantly lower in the groups exposed to SS after 15 d. Furthermore, there were obvious signs of DNA damage in these groups. We observed some recovery in these indices following transfer to clean seawater. However, recovery was incomplete and levels did not return to those of the controls. Our results suggest that these physiological and biochemical indices may be more sensitive than survival as indicators of exposure to increased SS in .

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李云,沈盎绿.悬沙胁迫下熊本牡蛎的损伤及恢复[J].中国水产科学,2012,19(1):138-144
LI Yun, SHEN Anglu. Damage and recovery in the Kumamoto oyster Crassostrea sikamea stressed by suspended solids[J]. Journal of Fishery Sciences of China,2012,19(1):138-144

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  • 在线发布日期: 2012-02-02
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