) is an important marine flatfish with significant potential for in China. This species exhibits a typical sexual dimorphism in which males grow significantly slower and smaller than females. Thus, there is little economic benefit to culturing males. To improve our understanding of this sex-associated dimorphic growth phenomenon, we screened differentially expressed genes from the pituitary tissues of female and male half-smooth tongue sole using the Affymetrix zebrafishmicroarray. d. Based on standards for the relative strength of the difference (i.e., a ratio of the male to female hybridization signals of >1.5 or <0.6), we identifed 39 differentially expressed genes, including col1a2, slc25a5, . Our results provide a foundation for investigating the molecular mechanisms controlling differential growth among the sexes.