We used native-PAGE and virus overlay protein binding assay (VOPBA) to identify binding proteins for lymphocystis disease virus in flounder() gill cells(FG). We isolated a 135 kD putative LCDV binding protein molecule. SDS-PAGE and two-dimensional (2-D) gel electrophoresis analysis of this molecule indicated it consisted of three proteins with molecular weights of 37.6 kD polypeptide was able to bind to LCDV.