1. 山东农业大学 动物科技学院, 山东 泰安, 271000;2. 山东省淡水水产研究所, 山东 济南, 252000;3. 泰山医学院, 山东 泰安, 271000
刘含亮(1986−) , 男, 硕士研究生, 研究方向为鱼类营养与免疫. E-mail: liuhanliang666@163.com
S917
山东省自然科学基金项目(Y2008D35); 山东省高等学校科技计划项目(J09LC05).
1. College of Animal Science and Technology, Shandong Agricultural University, Taian 271000, China; 2. Freshwater Fishery Research Institute of Shandong Province, Jinan 252000, China; 3. Department of Basic Medicine, Taishan Medical College, Taian 271000,
We evaluated the effect of glucose injection on and glycolytic enzymes in GIFT (=150, mean weight 80±5 g) wererandomly assigned to one of two groups, a control group that was injected with sterile 0.7% saline and a treatment group that was injected intraperitoneally with glucose (30 mg/100 g wet weight). The fish in both groups were euthanized 1–12 h after injection. Glucose injection resulted in hyperglycemia with maximum glucose levels observed 1 h after injection (<0.05) and a return to normal levels after 3 h. Cholesterol content also increased significantly (<0.05) after glucose injection. Conversely, triglycerides, glutamic-pyruvic transaminase (GPT) levels were unaffected by glucose injection. The level of glutamic-oxalacetic transaminase (GOT) decreased significantly (<0.05) after injection. Liver glycogen peaked 6 h after injection with glucose, then decreased significantly (<0.05). Glucose injection had no effect on muscle glycogen levels. Insulin levels increased significantly (<0.05) in the plasma and muscle, but not in the liver, following glucose injection. Pyruvate kinase levels peaked 6 h after glucose injection (<0.05). Conversely, there was no change in hexokinase activity after injection. Our results suggest that metabolic adjustment caused by glucose administration is reflective of the regulatory action of insulin and pyruvate kinase. The levels of insulin and pyruvate kinase (PK) were unable to maintain pace with the absorption speed of carbohydrates in fish and a shortage in hexokinase (HK) activity limited glucose utilization, leading to higher concentrations of cholesterol.
刘含亮,孙敏敏,王红卫,付佩胜,周庆杰,万文菊,王纪亭.注射葡萄糖对吉富罗非鱼血浆生化指标、胰岛素和糖酵解关键酶的影响[J].中国水产科学,2012,19(5):813-820
LIU Hanliang, SUN Minmin, WANG Hongwei, FU Peisheng, ZHOU Qingjie, WAN Wenju, WANG Jiting. Effects of glucose injection on biochemical parameters, insulin, and glycolytic enzymes in GIFT (Oreochromis niloticus)[J]. Journal of Fishery Sciences of China,2012,19(5):813-820
