1. 苏州大学 基础医学与生物科学学院, 苏州 215123; 2. 江苏省水生动物疫病预防控制中心, 南京210036; 3. 南京师范大学 生命科学学院, 南京 210036
刘林(1986−), 女, 在读硕士研究生, 研究方向为基因工程与生物制药.
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江苏省科技支撑计划项目(BE2009387); 苏州市自然科学基金(SYN201106); 江苏省农业科技自主创新资金项目(SCX(11)2165).
1. Pre-clinical Medical and Biological Science College, Soochow University, Suzhou 215123, China; 2. Jiangsu Center for Control and Prevention of Aquatic Animal Infectious Disease, Nanjing 210036, China; 3. College of Life Sciences, Nanjing Normal Univers
DNA vaccine, We constructed a baculovirus transfer vector pFastBac™Dual-derived DNA vaccine vector pFastBac-FA-VP6-ph-VP6 in which one gene was controlled by the baculovirus polyhedron (Ph) promoter, and another -actin promoter of grass carp, at the downstream of the baculovirus p10 promoter. Grass carp (weight 60−120 g, length 14−20 cm) were injected with 10, 30, or 60 μg of the vaccine vector, the negative control group was inject with 30 μg of pFastBac™Dual, and the unvaccinated control group was injected with 0.4 mL of sterile water. We then analyzed the pattern of expression in vaccinated fish using RT-PCR, measured antibody titers in the blood by indirect agglutination at 14, 21, 28, 49, and 70 d post-vaccination and evaluated immune efficacy by challenging GCRV at 21 d post-vaccination. The specific antibodies were detected in all vaccinated groups, and its levels peaked at 28th day after immunization. The mortalities were 0, 0, and 5% in the vaccinated groups injected with 10, 30, and 60 μg of DNA vaccine, and 30 % and 100% in the negative control group and the unvaccinated control group, respectively. Our results suggest that the DNA vaccine had strongly immunoprotective efficacy against GCRV.
刘林,徐诗英,李婧慧,邹勇,倪金俤,杨鸢劼,曹广力,.草鱼出血病病毒vp6核酸疫苗的免疫效果评估[J].中国水产科学,2012,19(5):841-847
LIU Lin, XU Shiying, LI Jinghui, ZOU Yong, NI Jindi, YANG Yuanjie, CAO Guangli, XUE Renyu, CHEN Hui, GONG Chengliang. Evaluation of immune efficacy of GCRV vp6 DNA vaccine[J]. Journal of Fishery Sciences of China,2012,19(5):841-847
