草鱼C1qC基因的克隆及表达分析
作者:
作者单位:

1. 上海海洋大学 水产动物种质资源挖掘与利用教育部重点实验室, 上海201306;

作者简介:

陈玥(1988−), 女, 硕士研究生, 研究方向为水产动物种质资源与遗传育种. Tel: 86-21-61900438; E-mail: doveyy88@ 163.com

中图分类号:

S917

基金项目:

现代农业产业技术体系建设专项资金项目(CARS-46-04); 国家科技支撑计划项目(2012BAD26B02); 上海市重点学科建设项目(Y1101).


Cloning and expression of C1qC gene in grass carp (Ctenopharyngodon idella)
Author:
Affiliation:

1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;

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    摘要:

    达到(grass carp reovirus, GCRV)C1qC表达水平均显著上调。在草鱼胚胎发育的各个阶段都能检测到

    Abstract:

    Ctenopharyngodon idella). The full-length cDNA was successfully cloned by RT-PCR and rapid amplification of cDNA ends (RACE). The full-length cDNA was 916 bp, consisting of a 735 bp open reading frame (ORF) encoding 244 amino acids, a 89 bp 5′untranslated region (5′ UTR), and a 92 bp 3′ untranslated region (3′ UTR). The molecular weight of this mature peptide was estimated to be 26 162.5U. Multiple alignment analysis revealed that grass carp C1qC shares the highest identity with zebrafish C1qC (71%). We constructed a phylogenetic tree using the neighbor-Joining (NJ) method. Grass carp were grouped most closely with zebrafish ( was significantly up-regulated in most tissues following challenge with grass carp reovirus (GCRV). The expression of expression differed during the various stages of embryonic development. Our results suggest that plays an important role in early embryonic development and in the response to GCRV-related diseases in grass carp. Our result provide a basis for further evaluation of the role of grass carp in immune function.

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引用本文

陈玥,李家乐,沈玉帮.草鱼C1qC基因的克隆及表达分析[J].中国水产科学,2013,20(1):25-34
CHEN Yue, LI Jiale, SHEN Yubang. Cloning and expression of C1qC gene in grass carp (Ctenopharyngodon idella)[J]. Journal of Fishery Sciences of China,2013,20(1):25-34

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  • 在线发布日期: 2013-02-06
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