technology. We determined that the titer of the primary cDNA library was 2.8 × 10⁶ pfu/mL, the recombinant efficiencies were over 99%, the insert size of the library was between 400 bp and 2 000 bp, and the tpfu/mL. One hundred single clones were selected randomly to screen for primary detection and a total of 95 high. The ESTs were into 70 unigenes, comprising 11 contigs and 59 singlets. When compared with the public database by Blast, 20 unigenes -value < 1e-5) to knownwere unknown genes (hypothetical protein), and 48 homology to any sequence. Several functional genes of Troponin I, Enolase, Anti-lipopolysaccharide factor and Lysozyme, were identified by homology comparison. The quality of this haemocyte cDNA library is of a high enough standard to obtain haemocyte-specific expression gene information. lts provide important information on functional genes and lay the groundwork for screening of molecular markers in .