胭脂鱼受精过程的细胞学观察
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淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室, 西南大学 生命科学学院,重庆400715

作者简介:

李萍(1981), 女, 博士研究生, 从事渔业生物学研究. E-mail: liping8111@yahoo.com.cn

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S965

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重庆市自然科学基金重点项目(CSTS, 2009BA1053).


Cytological study on fertilization process of Myxocyprinus asiaticus
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Key Laboratory of Aquatic Science of Chongqing, School of Life Science,Key Laboratory of Freshwater Fish Reproduction and Development, Education of Ministry, Southwest University, Chongqing 400715

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    摘要:

    , )短孔道型。在水温条件下精子开始穿过精孔管进入卵子形成精子星光卵子处于第二次减数分裂后期第二极体形成雌雄原核融合受精卵进入第一次有丝分裂中期第一次有丝分裂结束成熟卵子精孔器内无精孔细胞及其他物质精孔管内径; 这些结构特点提示其是造成胭脂鱼单精受精的原因之一。本研究旨在为胭脂鱼人工繁殖技术的研究及其保护管理提供基础资料。

    Abstract:

    egg was studied using artificial fertilization, histology, ultrastructural technology methods etc. The observation showed that there is only one micropyle in shell of egg’s animal pole of belongs to the type of deep concave and short channel.At water temperature of 17sperm passed through micropylar apparatus at 3 s after insemination and entered ovum at 5 s. Sperm star light was formed at 10 s after insemination. After 15 min after insemination ovum was in late time of second meiotic division and second polar body appeared. Ovum was in telophase after 20 min and second meiotic division was formed. Male and female pronucleus was fused after 90 min. vum was in metaphase of first karyokinesis at 140 min after insemination. At 180 min after insemination first karyokinesis was over and second cell was formed. The results showed that in micropylar apparatus of mature egg there is no micropylar cell and other stuff, which is similar to that of most other teleost. The inner diameter of micorpylar apparatus is 2.42 µm and near to the diameter of sperm head. There are many spines in inner walls of anterior micropylar apparatus and holes are arranged in spiral way, which was one of the reasons for monospermy. This study aims to provide basic data for the research of artificial reproduction technology of .

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李萍,刘晓蕾,李勇,江星,金丽,张耀光.胭脂鱼受精过程的细胞学观察[J].中国水产科学,2013,20(2):250-259
LI Ping, LIU Xiaolei, LI Yong, JIANG Xing, JIN Li, ZHANG Yaoguang. Cytological study on fertilization process of Myxocyprinus asiaticus[J]. Journal of Fishery Sciences of China,2013,20(2):250-259

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  • 在线发布日期: 2013-04-03
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