The ciliated olfactory receptor neurons (cORNs) in the olfactory sac were dissociated from by enzymatic and mechanical methods. The whole-cell patch-clamp technique was used to determine the resting potential, action potential and voltage-gated ion channel current. The results showed that the cORN was a bipolar neuron, containing cell body, axon and dendrites with olfactory knobs and cilia. The resting and action potential peaks of ciliated olfactory receptor neurons were (–57 ± 6.37) mV and (38.30 ± 0.95) mV, respectively. In response to depolarizing voltage steps pulse stimulation from –60 ~ +80 mV, the transient inward current was activated between – pA at +40 mV. The present study provides a base theory for research in the electrophysiological mechanisms of sex pheromones with their receptors and the application of sex pheromones.