1. 中国水产科学研究院 东海水产研究所, 农业部海洋与河口渔业资源与生态重点开放实验室, 上海 200090;2. 华东师范大学 生命科学学院, 上海 200062;3. 上海海洋大学 水产与生命学院, 上海 201306
王瑞芳(1984), 博士研究生, 主要从事水生动物生理生态学与行为生态学研究. E-mail: RFWang019@gmail.com
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国家973计划项目(2010CB429005); 国家公益性行业(农业)科研专项(200903048-07, 201203065); 中央级公益性科研院所基本科研业务费专项(东海水产研究所2011M08).
1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, Shanghai 200090, China;2. School of Life Science, East China Nor
<0.05)低渗胁迫引起胚胎蛋白含量降低而高渗胁迫导致酶活性降低渗透胁迫对原肠期胚胎消化酶活性的影响均具有显著性, 渗透胁迫通过改变中华绒螯蟹胚胎消化酶的活性进而影响胚胎对卵黄物质的分解与利用
, is completed in estuaries where they are exposed to frequent and abrupt changes in environmental salinity. Biochemical composition (carbohydrates, proteins and lipids) content and digestive enzyme (amylase, lipase, pepsin and trypsin) activity were determined in three developmental stages (gastrula, eyespot and pre-hatching stage) of eggs under hyper- or hypo-osmotic stress. Increases in pepsin and trypsin activity (<0.05), lipid content (<0.05) in the eggs of the control group were noticed during embryonic development. Total carbohydrate content and amylase activity increased at the eyespot stage (<0.05). The protein content during all three developmental stages decreased under hyper-osmotic stress while it increased under hypo-osmotic stress (<0.05). Total lipid content was unaffected by hyper-osmotic stress at any developmental stage, whereas it reduced significantly at the pre-hatching stage under hypo-osmotic stress (<0.05). Total carbohydrate content did not show any significant variation after osmotic stress. Hyper-osmotic stress reduced enzyme activity at the three developmental stages; however, hypo-osmotic stress enhanced it, with the exception of reducing amylase activity in the eyespot stage (<0.05) and trypsin activity in the pre-hatching stage under both hyper- and hypo-osmotic stress (>0.05).effect of salinity changes on digestive enzymes was only statistically significant during the gastrula stage (<0.05). We suggest that proteins and lipids are important structural materials of embryonic development and provide energy for this process. Osmotic stress changed the activity of digestive enzymes, which further affects the use of yolk substances, and consequently affects embryonic development. or low salinity in estuaries potentially affects the embryonic development of, especially under salt-water encroachment.
王瑞芳,庄平,黄晓荣,冯广朋,章龙珍,陆建学,王海华,王妤.渗透胁迫对中华绒螯蟹胚胎生化组成及消化酶活性的影响[J].中国水产科学,2013,20(2):299-307
WANG Ruifang, ZHUANG Ping, HUANG Xiaorong, FENG Guangpeng, ZHANG Longzhen,. Developmental changes of biochemical composition and digestive enzyme activity in the eggs of the Chinese mitten crab, Eriocheir sinensis, under osmotic stress[J]. Journal of Fishery Sciences of China,2013,20(2):299-307
