(♂) using microsatellite markers. We selected 21 loci with different-sized alleles in from 86 pairs of microsatellites. These loci contained 59 alleles, including 22 specific alleles in . The observed heterozygosity was higher in the F₁ (=0.739) generation. Conversely, the effective number of alleles(2 were similar to those in F₁(=2.45, PIC=0.489). Heterozygous alleles were observed at all loci in the F₁, whereas we found evidence of allelic separation at F₂ loci. Eighteen loci followed strict Mendelian segregation (chi-squared: 1>) exhibited segregation distortion () exhibited non-segregation. Among the 18 Mendelian loci, the F₂ possessed the similar allelic frequencies for specific alleles (47.7%). The genotype frequencies of F₁ type, type observed iwere 58.2%, 19.9%, and 21.9%, respectively. Our results suggest that the F₂ grossly inherited the heterozygosity from F₁, but that there is also a certain degree of separation.