野生和“黄海1号”中国明对虾不同组织基因组DNA的MSAP分析
作者:
作者单位:

1. 中国海洋大学 海洋生命学院, 山东 青岛 266003;2. 中国水产科学研究院 黄海水产研究所, 山东 青岛 266071;3. 中国海洋大学 水产学院, 山东 青岛266003

作者简介:

杜盈(1987−), 女, 硕士研究生, 研究方向为鱼类遗传育种. E-mail: duying1226@163.com

中图分类号:

S917

基金项目:

国家自然科学基金项目(31172401); 国家虾产业技术体系项目(CARS-47); 科技部农业科技成果转化基金项目(2010 GB23260589).


MSAP analysis of genomic DNA in the tissues of wild and “Huanghai No.1” Fenneropenaeus chinensis
Author:
Affiliation:

1. College of Marine Life Sciences, Ocean University of China; Qingdao 266003, China;2. Yellow Sea Fisheries Research Institute, Qingdao 266071, China;3. Chinese Academy of Fishery Sciences, Ocean University of China, Qingdao266003, China

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    摘要:

    (mehylation sensitive amplified polymorphism, MSAP)肌肉、鳃、血液的对引物进行选择性扩增(Polyacrylamide Gel 带型结果显示23.1%, 22.3%121.4%18.9%号”同一组织间的甲基化水平和甲基化多态性水平不同间变化趋势最大中国明对虾生长性状间的相关性研究提供依据。

    Abstract:

    . We used MSAP (Methylation-Sensitive Amplification Polymorphism) to analyze methylation patterns of genomic DNA in the muscle, gill, and blood of wild and cultivated “Huanghai No.1”, a strain selected for faster growth rates and improved disease resistance. DNA methylation is closely linked to biological events, including chromatin inactivation, transgene silencing, genomic imprinting, and control of parasitic DNA elements. Because of its efficiency and competence, the MSAP technique has been increasingly used in genomic DNA or individual functional genes studies to analyze DNA methylation levels. We used 30 pairs of selective-amplification primers. Using polyacrylamide gel electrophoresis, we estimated the methylation ratios in the muscle, gill, and blood of wild stock were 23.1%, 22.3%, and 19.7%, respectively, and 21.4%, 19.6%, and 18.9%, respectively, in cultured shrimps. These levels are much lower than in some plants and animals, perhapsbecause of differences in the amount of genomic DNA. The methylation levels at the CCGG site differed among different tissues of the same stock and between the same tissue among different stocks. The level of methylation in genomic DNA was always higher in the organs than in the blood. We observed variation in the DNA polymorphic methylation between wild and “Huanghai No.1” stocks in the blood and gill, but not in the muscle. Furthermore, polymorphic methylation was associated with demethylation and methylation of CCGG loci. Additional research is needed to understand the biological meaning of variation in methylation among CCGG loci. Our approach provides a basis for further studies on the mechanism of methylation-mediated DNA methyltransferases in .

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引用本文

杜盈,何玉英,李健,刘磊,孙铭,王清印.野生和“黄海1号”中国明对虾不同组织基因组DNA的MSAP分析[J].中国水产科学,2013,20(3):536-543
DU Ying, HE Yuying, LI Jian, LIU Lei, SUN Ming, WANG Qingyin. MSAP analysis of genomic DNA in the tissues of wild and “Huanghai No.1” Fenneropenaeus chinensis[J]. Journal of Fishery Sciences of China,2013,20(3):536-543

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  • 在线发布日期: 2013-05-29
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