; testosterone, T), protein phosphorus (SPP), and protein calcium (SPC). In addition, we quantified ) mRNA expression in the liver and ovary. We observed phase ℃did not develop beyond phase II by the end of the experiment (6 weeks). The majority of the indices increased with treatment time, except the hepatosomatic index (HSI). Temperature had no effect on the levels of testosterone. The gonadosomatic index (GSI) and E₂ levels were significantly elevated in fish held at 23℃<0.05). The levels of SPP were higher in fish held at 23°C and 26 group, but the difference was only significant for the group held at 23mRNA expression was lower in the ovary than in the liver. The effect of temperature on liver levels. However, ovarian mRNA expression was only significantly higher in the 26 group. In summary, the optimal temperature for the ovarian development of . In addition, there is a significant positive correlation between liver content