大眼鳜与翘嘴鳜正反交及其正交子代自交的胚胎发育观察
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1. 水产品安全教育部重点实验室, 水生经济动物研究所, 中山大学 生命科学学院, 广东 广州 510275;2. 生生农业集团股份有限公司, 广东 佛山 528300

作者简介:

卢薛(1986−), 男, 博士研究生, 主要从事鱼类生态与资源保护研究. E-mail: luxue1986@aliyun.com

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S917

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广东省战略性新兴产业核心技术攻关项目(2012A020800001); 广东省教育厅项目(cxzd1104); 农业科技成果转化资金项目(2012GB2E000338); 广东省科技计划项目(2008A020100003, 2007A020300001-1, 2007B020503006); 广东省教育部产学研结合项目(2011B090400179).


Observations on embryonic development of reciprocal hybrids of Siniperca kneri Garman × Siniperca chuatsi Basilewsky and F2 of S. kneri females × S. chuatsi males F1
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1. MOE Key Laboratory of Aquatic Product Safety, Institute of Aquatic Economic Animals, College of Life Science, SunYat-senUniversity, Guangdong, Guangzhou 510275, China;2. Shengsheng Agriculture Group Co. Ltd., Foshan 528300, China

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    摘要:

    )(♀)×正交大眼鳜、大眼鳜正交的胚胎发育进行观察(1.197±0.052) mm, (1.723±0.0519) mm(<0.05)25.5~27.7, F2(4.1±0.4) mm(3.5±0.2) mm3并在油球处有集中现象21.6~28.7在水温与大眼鳜的胚胎发育时间有较大差别。

    Abstract:

    are two economically important freshwater aquaculture species in China. Hybrids created by interspecific crossing among several fish exhibit increased growth rate, improved disease resistance, and improved environmental tolerance. In this study, the embryonic development of reciprocal hybrids of Basilewsky (DQ and QD) and F2 of (♂) F1 (F2) were described and compared to each other and their parents. Embryonic development was divided into 6 main periods based on anatomical featureszygote, cleavage, blastula, gastrula, embryonic body formation, and hatching. S. chuatsi, with a mean diameter of (1.197±0.052) mm, were both significantly bigger than that of the F1 ( males) with 1.723±0.0519) mm. Compared with the early development of other fish, e.g., , and zebrafish, we found that chromatogenesis and pigment distribution in , and the three hybrids shared common characteristics: chromatogenesis began on the yolk sac just after the closure of the blastopore. Punctiform melanin gradually spread all over the surface of the yolk sac. A neurocyte-shaped pigment subsequently centralized on the oil globule, and pigmentation later appeared on eyes and head. The features of embryonic development in the three hybrids were similar to their parents. DQ began to hatch 37 h post-fertiliza­tion at 25.5‒27.7 began to hatch 35 h, 34 h, 33 h, 31 h post-fertilization at 25, 26, 27, and 27.5, respectively. The speed of DQ, QD, and F2 embryonic development at 21.6‒28.7. Newly hatched DQ, QD, and F2 larvae were (4.1±0.4) mm3.5±0.2) mm in length, respectively. The similarity in embryonic development of the three hybrids and their parents, and the progeny of males both indicate a very close phylogenetic relationship between , which is consistent with previous studies employing 11 nuclear loci and the cytochrome b gene.

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卢薛,孙际佳,王海芳,罗渡,侯晓翠,刘凌志,李桂峰.大眼鳜与翘嘴鳜正反交及其正交子代自交的胚胎发育观察[J].中国水产科学,2013,20(5):975-981
LU Xue, SUN Jijia, WANG Haifang, LUO Du, HOU Xiaocui, LIU Lingzhi, LI Guifeng. Observations on embryonic development of reciprocal hybrids of Siniperca kneri Garman × Siniperca chuatsi Basilewsky and F2 of S. kneri females × S. chuatsi males F1[J]. Journal of Fishery Sciences of China,2013,20(5):975-981

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  • 在线发布日期: 2013-09-18
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