1. 集美大学 水产学院, 福建省高校水产科技与食品安全重点实验室, 福建 厦门 361021;2. 厦门大学 海洋与地球学院, 福建 厦门 361005
林桂芳(1988−), 女, 硕士研究生, 主要从事水产微生物研究. E-mail: grafang@163.com
S941
国家自然科学基金项目(31272699); 福建省自然科学基金项目(2011J06014).
1. Fisheries College, Jimei University; Key Laboratory of Science and Technology for Aquaculture and Food Safety, Xiamen 361021, China;2. College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China
嗜水气单胞菌黏附鳗鲡表皮黏液的最佳条件为15~25 g/L150 min葡萄糖和麦芽糖能显著提高嗜水气单胞菌的黏附量, 且其黏附作用具有可控性。
Adhesion to the host mucus, which is closely related to the virulence of pathogenic bacteria, is the first crucial step in most infections. In this paper, the adhesion characteristics of ) skin mucus was investigated by indirect ELISA. can be used as an index to reflect the number of adhering bacteria. Second, we confirmed that the iCFU. The number of a saturation kinetics equation =0.986) was obtained by plotting the number of adhering bacteria against cell density. The factors affecting bacterial adhesion were then investigated. The results revealed that the number of adhering increased with incubation time and reached a peak after 150 min. Temperature was one of the major factors affecting bacterial adhesion, with peak adhesion occurring at 20−28. pH also influenced bacterial adhesion. In a weak acid with a pH of 6.2−6.6, the adhesion ability of The optimum concentrations of Na+ and adhesion were 1.5−2.5 g/L and 3 g/L, respectively. In summary, the optimum conditions for the adhesion of to eel skin mucuswere 20‒28, and 150 min incubation time. In addition, we found that bacterial adhesion was influenced by carbohydrates. Seven kinds of carbohydrates were investigated and the results indicate that glucosehelp to further elucidate bacterial pathogenicity and help
