半滑舌鳎Dmrt1蛋白表达、纯化及功能
作者:
作者单位:

1. 中国水产科学研究院 黄海水产研究所, 山东 青岛 266071;2.上海海洋大学, 上海 201306

作者简介:

胡乔木(1984-), 男, 博士研究生, 主要从事水产养殖研究

中图分类号:

Q959

基金项目:

国家863计划项目(2012AA092203); 国家自然科学基金项目(31130057, 31072202); 山东省泰山学者工程专项资助


Protein expression, purification, and elementary function of Dmrt1 in half-smooth tongue sole (Cynoglossus semilaevis)
Author:
Affiliation:

1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

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    摘要:

    PCR~24 h48 h,

    Abstract:

    C. semilaevis Dmrt1 was expressed by the prokaryotic expression system (Pet-32a) and the protein (Pet-32-dmrt1) was subsequently purified by immobilized-metal affinity chromatography. We measured gene expression after injection of the recombinant protein Pet-32-Dmrt1 into the ovary. The expression of CYP19A and Foxl2 decreased significantly between 6 and 24 h after injection and the mRNA transcription level of Sox9aa was significantly increased between 6 and 24 h. However, the expression of all three genes expression returned to normal 48 h after injection. Our results indicate that recombinant protein has biological activity, and plays a role in sex differentiation by regulating sex related gene expression.

    参考文献

           Koopman P. The genetics and biology of vertebrate sex determination [J]. Cell, 2001, 105: 843-847.

           Jorgensen A, Morthorst J, Andersen O, et al. Expression profiles for six zebrafish genes during gonadal sex differentiation[J]. Reprod Biol Endocrinol, 2008(6): 25.

           Kobayashi T, Kajiura-Kobayashi H, Guan G, et al.Sexual dimorphic expression of dmrt1 and Sox9a duringgonadal differentiation and hormone-induced sex reversalin the teleost fish Nile tilapia (Oreochromis niloticus) [J]. Dev Dynam, 2008, 237: 297-

           Alam M A, Kobayashi Y, Horiguchi R, et al. Molecular cloning and quantitative expression of sexually dimorphic markers Dmrt1 and Foxl2 during female-to-male sex change in Epinephelu smerra [J]. Gen Comp Endocr, 2008, 157: 75-

           文爱韵尤锋孙鹏牙鲆基因的克隆及其与基因的组织表达分析海洋科学-

           邓思平陈松林半滑舌鳎基因的克隆及其表达中国水产科学-

           李林梁宏伟李忠黄颡鱼基因全长克隆及其表达分析华中农业大学学报-

           邓思平王静杰吴天利胡子鲇基因全长克隆及其表达分析水生生物学报-

           Cao M X, Duan J D, Cheng N N, et al. Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobio cyprisrarus[J]. Compar Biochemi Physiol, Part A, 2012, 162: 303–309.

        Nanda, I, Shan Z, Schartl M, et al. 300 million years of conserved synteny between chicken Z and human chromosome 9[J]. Nat Genet, 1999, 21: 258-

        Smith C A, Roeszler K N, Ohnesorg T, et al.The avian Z-linked gene DMRT1 is required for malesex determination in the chicken[J].Nature, 2009, 461: 267-

        邓景耀孟田湘任胜民渤海鱼类种类组成及数量分布海洋水产研究-

        Deng S P, Chen S L, Liu B W, et al, Molecular cloning, characterization and expression analysis of gonadal CYP19A aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis[J]. Aquaculture, 2008(10): 1-

        Dong X L, Chen S L, Ji X S, etal.Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossus semilaevis)[J]. Acta Oceanol Sin, 2011, 30(1): 68-

        Matsuoka M P, Van Nes S, Andersen O, et al. Real-timePCR analysis of ovary-and brain-type aromatase gene expression during Atlantic halibut (Hippoglossus hippoglossus) development [J]. Compar Biochemi Physiol, Part B: Bioch Molec Biol, 2006, 144: 128-

        Zhang Y, Li H M, Sun J, et al. DC-Chol/DOPE cationic liposomes: A comparative study of the influence factorson plasmid pDNA and siRNA gene delivery[J]. Intern J Pharm, 2010, 390: 198-

        Matson C K, Murphy M W, Sarver A L, et al. DMRT1 prevents female reprogramming in the postnatal mammalian testis[J]. Nature, 2011, 476: 101-

        Matsuda M, Nagahama Y, Shinomiya A, et al. DMY is aY-specific DM-domain gene required for male developmentin the medaka fish [J]. Nature, 2002, 417: 559-

        Zhou H Y, Wu S L, Joo J Y, et al. Generation of induced pluripotent stem cells using recombinant proteins[J].Cell Stem Cell, 2009(4): 1-

        李铮刘君炎两种方法制备阳离子脂质体包裹蛋白转染法的比较华中医学杂志-

        Ignatius R, Mahnke K, Rivera M, et al. Presentation of proteins encapsulated in sterically stabilized liposomesby dendritic cells initiates CD8 + T-cell responses in vivo[J]. Blood, 2000, 96(10): 3505-

        Zhou F T, Rouse B, Huang L. Induction of cytotoxic T lymphocytes in vivo with protein antigen entrapped in membranous vehicles[J]. J Immunol, 1992, 149: 1599-

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胡乔木,王凯琳,陈松林.半滑舌鳎Dmrt1蛋白表达、纯化及功能[J].中国水产科学,2013,20(6):1132-1138
HU Qiaomu, WANG Kailin, CHEN Songlin. Protein expression, purification, and elementary function of Dmrt1 in half-smooth tongue sole (Cynoglossus semilaevis)[J]. Journal of Fishery Sciences of China,2013,20(6):1132-1138

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  • 在线发布日期: 2013-11-28
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